Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 02, 2021

Anti-EB3 antibody [EPR11421(B)] (ab157217)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11421(B)] to EB3
Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
Reacts with: Rat, Human

Product name

Anti-EB3 antibody [EPR11421(B)]
See all EB3 primary antibodies
Description

Rabbit monoclonal [EPR11421(B)] to EB3
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human EB3. The exact sequence is proprietary.
Positive control
- WB: Human cerebellum, human fetal brain and rat muscle lysates. IHC-P: Human brain tissue, Human skeletal muscle tissue. Flow Cyt: U87 MG cells. ICC/IF: SH-SY5Y cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11421(B)
Isotype

IgG
Research areas

Western blot - Anti-EB3 antibody [EPR11421(B)] (ab157217)

All lanes : Anti-EB3 antibody [EPR11421(B)] (ab157217) at 1/10000 dilution

Lane 1 : Human cerebellum lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Rat muscle lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 32 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EB3 antibody [EPR11421(B)] (ab157217)

Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labeling EB3 with purified ab157217 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control: PBS only.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] (ab157217)

Immunohistochemical analysis of paraffin embedded human skeletal muscle tissue labeling EB3 with ab157217 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-EB3 antibody [EPR11421(B)] (ab157217)

Flow Cytometry analysis of U87-MG (human glioblastoma) cells labeling EB3 with unpurified ab157217 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EB3 antibody [EPR11421(B)] (ab157217)

Immunohistochemical analysis of paraffin embedded human brain tissue labeling EB3 with ab157217 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-EB3 antibody [EPR11421(B)] (ab157217)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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