Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21057-141] to STAT1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ChIP, WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free
    See all STAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR21057-141] to STAT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ChIP, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, HEK-293T and MCF7 cell lysate; Human heart tissue lysate. IHC-P: Human tonsil and kidney tissue; Paried human endometrial cancer and non-tumour endometrium tissue. Flow Cyt (intra): HeLa and MCF7 cells. IP: MCF7 whole cell lysate. ChIP1: HeLa cells.

  • General notes

    ab234904 is the carrier-free version of ab234400.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Western blot - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    All lanes : Anti-STAT1 antibody [EPR21057-141] - ChIP Grade (ab234400) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : STA1 knockout HAP1 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 87 kDa



    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

    Lanes 1 - 3: Merged signal (red and green). Green - ab234400 observed at 87 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab234400 was shown to specifically react with STAT1 in wild-type HAP1 cells as signal was lost in STA1 knockout cells. Wild-type and STA1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab234400 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • ChIP - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    ChIP - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

    Chromatin was prepared from HeLa (starve overnight) + hIFN-α (serum-starved overnight) (1,000 units/ml,30 min) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 μg of chromatin, 2 μg of ab234400 (red), and 20 µl of protein A/G sepharose beads slurry (10 µl of sepharose A beads + 10 µl of sepharose G beads). 2 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR approach).
    ChIP results are consistent with the literature (PMID: 16319195).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

  • Immunoprecipitation - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Immunoprecipitation - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

    STAT1 was immunoprecipitated from 0.35 mg of MCF7 (human breast adenocarcinoma cell line) whole cell lysate with ab234400 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab234400 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
    Lane 1: MCF7 whole cell lysate 10 μg (input).
    Lane 2: ab234400 IP in MCF7 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab234400 in MCF7 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The doublet observed in some lanes likely represent the α and β isoforms of STAT1 (PMID: 8647800).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

  • Flow Cytometry - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Flow Cytometry - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized MCF7 (human breast adenocarcinoma cell line) cells labeling STAT1 with ab234400 at 1/500 (Red) compared with Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

  • Flow Cytometry - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Flow Cytometry - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling STAT1 with ab234400 at 1/500 (Red) compared with Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

    Immunohistochemical analysis of paraffin-embedded paired human endometrial cancer (A) and non-tumor endometrium tissue (B) labeling STAT1 with ab234400 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Much higher staining intensity of endometrial cancer (A) than its paired non-tumor endometrium (B) (PMID: 25267067) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling STAT1 with ab234400 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on glomerulus and some renal tubules of human kidney (PMID: 26678048) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling STAT1 with ab234400 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining mainly on interfollicular cells of human tonsil (PMID: 25336386; PMID: 25921060) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234400).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)
    Anti-STAT1 antibody [EPR21057-141] - BSA and Azide free (ab234904)

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