Antibodies, Proteins, Kits and Reagents for Life Science

247 929 ₸ Product size

100 µl 247 929 ₸

Order now and get it on Tuesday March 16, 2021

Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3147] to STAT1 (phospho Y701)
Suitable for: WB, Dot blot
Reacts with: Human

Product name

Anti-STAT1 (phospho Y701) antibody [EPR3147]
See all STAT1 primary antibodies
Description

Rabbit monoclonal [EPR3147] to STAT1 (phospho Y701)
Host species

Rabbit
Specificity

This antibody only detects STAT1 when phosphorylated at Tyrosine 701.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A431 cell lysate.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR3147
Isotype

IgG
Research areas

Dot Blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

Primary antibody dilution: 1/1000

Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

Secondary antibody dilution: 1/2500

Blocking & dilution buffer: 5% NFDM/TBST

Lane 1 sample: STAT1 (pY701) phospho peptide

Lane 2 sample: STAT1 non-phospho peptide

Exposure time: 3 minutes
Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

All lanes : Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

Lane 1 : A431 (human epidermoid carcinoma) treated with Epidermal Growth Factor (EGF) whole cell lysates
Lane 2 : Untreated A431 (human epidermoid carcinoma) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 87 kDa
Observed band size: 91 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM /TBST
Western blot - Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

All lanes : Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457) at 1/1000 dilution

Lane 1 : A431 cell lysates untreated with Epidermal Growth Factor (EGF).
Lane 2 : A431 cell lysates treated with Epidermal Growth Factor (EGF).

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 87 kDa

The loading control is Anti-stat1 antibody, ab92506. 1/1000 dilution.
Anti-STAT1 (phospho Y701) antibody [EPR3147] (ab109457)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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