Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23049-111] to STAT1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ChIP, WB, ICC/IF, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free
    See all STAT1 primary antibodies

  • Description

    Rabbit monoclonal [EPR23049-111] to STAT1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ChIP, WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human lymph node, RAW264.7, HeLa, MCF7, Mouse lung, Mouse breast and NIH/3T3 lysates. ICC/IF: HeLa and RAW 264.7 cells. Flow Cyt (intra): RAW264.7 cells. IP: RAW264.7 cells.

  • General notes

    ab263866 is the carrier-free version of ab239360.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
    ChIP - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)

    Chromatin was prepared from HT-1080 cells treated with IFN-γ (50ng/ml 30min) according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.

    The  ChIP was performed with 25 µg of chromatin, 5 µg of  ab239360 (red), or 5 µg of rabbit normal IgG ab172730 (gray) and 20 µl of Protein A/G sepharose beads.  The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).

    Primers and probes are commercial primers from Cell Signaling Technology (Cat. No.: #5148)

    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239360).

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling STAT1 with ab239360 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1\1000 dilution (Green). Confocal image showing the signal translocate to nuclear in HeLa cells treated with IFN-γ (10ng/ml) for 30 mins. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239360).

  • Flow Cytometry - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
    Flow Cytometry - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling STAT1 with ab239360 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239360).

  • Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
    Immunoprecipitation - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)

    STAT1 was immunoprecipitated from 0.35mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab239360 at     1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239360 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate (10ug)

    Lane 2: ab239360 IP in RAW264.7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab239360 in RAW264.7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 75 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239360).

  • Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
    Immunocytochemistry/ Immunofluorescence - Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling STAT1 with ab239360 at 1/50 dilution, followed byab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing the signal translocate to nuclear in RAW 264.7 cells treated with IFN-γ (10ng/ml) for 30 mins. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239360).

  • Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)
    Anti-STAT1 antibody [EPR23049-111] - BSA and Azide free (ab263866)

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