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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)

Biotin Anti-CXCL1/GRO alpha antibody (ab271204)

Biotin Anti-CXCL1/GRO alpha antibody (ab271204)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Biotin Rabbit polyclonal to CXCL1/GRO alpha
  • Suitable for: WB, ELISA
  • Reacts with: Recombinant fragment
  • Conjugation: Biotin
  • Isotype: IgG

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Overview

  • Product name

    Biotin Anti-CXCL1/GRO alpha antibody
    See all CXCL1/GRO alpha primary antibodies
  • Description

    Biotin Rabbit polyclonal to CXCL1/GRO alpha
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Mouse CXCL1/GRO alpha aa 1-100.
    Database link: P12850

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Recombinant mouse CXCL1/GRO alpha protein.

Properties

  • Form

    Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml.
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage buffer

    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)

Images

  • Western blot - Biotin Anti-CXCL1/GRO alpha antibody (ab271204)
    Western blot - Biotin Anti-CXCL1/GRO alpha antibody (ab271204)

    To detect mouse CXCL1/GRO alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml.

    Used in conjunction with compatible secondary reagents the detection limit for recombinant mouse CXCL1/GRO alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    Lane 1: Marker

    Lanes 2-12: 250, 125, 62.5, 31.25, 15.625, 7.8, 3.9, 1.95, 0.975, 0.4875 and 0.24 ng recombinant mouse CXCL1/GRO alpha,  respectively.

    Non-reducing conditions.

  • ELISA - Biotin Anti-CXCL1/GRO alpha antibody (ab271204)
    ELISA - Biotin Anti-CXCL1/GRO alpha antibody (ab271204)

    Mouse CXCL!/GRO alpha was detected by sandwich ELISA (using 100 µl/well) using a concentration of 0.25-1.0 µg/ml of ab271204. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mouse CXCL1/GRO alpha protein.

    KC = CXCL1/GRO alpha

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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