Mouse CXCL15 ELISA Kit (ab234567)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.7 pg/ml
- Range: 25 pg/ml - 4800 pg/ml
- Sample type: Hep Plasma, Other biological fluids, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CXCL15 ELISA Kit
See all CXCL15 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Lung 8 2.5% Inter-assay Sample n Mean SD CV% Lung 3 3.9% -
Sample type
Other biological fluids, Tissue Extracts, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.7 pg/ml -
Range
25 pg/ml - 4800 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Other biological fluids 118 114% - 120% Tissue Extracts 108 108% - 109% Hep Plasma 91 91% - 93% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse CXCL15 ELISA Kit (ab234567) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL15 protein in other biological fluids, tissue extracts, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse CXCL15 with 1.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse CXCL15 Capture Antibody 1 x 600µl 10X Mouse CXCL15 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BR 1 x 6ml Mouse CXCL15 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic for neutrophils. Involved in lung-specific neutrophil trafficking during normal and inflammatory conditions. -
Tissue specificity
Expression restricted to the lung, produced by bronchoepithelial cells and is released into the airways. Expressed at low levels in fetal lung. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 15
- Chemokine (C-X-C motif) ligand 15
- Cxcl15
see all -
Database links
- Entrez Gene: 20309 Mouse
- SwissProt: Q9WVL7 Mouse
- Unigene: 64326 Mouse
Images
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Other - Mouse CXCL15 ELISA Kit (ab234567)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse CXCL15 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of mouse CXCL15 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CXCL15 in mouse plasma -heparin, lung supernatant and bronchoalveolar lavage samplesThe concentrations of CXCL15 were measured in duplicates, interpolated from the CXCL15 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma - heparin 50%, lung supernatant 10% and bronchoalveolar lavage 5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL15 concentration was determined to be 757.6 pg/mL in neat plasma – heparin, 13354.54 pg/mL in neat lung supernatant, and 5334.90 pg/mL in neat bronchoalveolar lavage.
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Interpolated concentrations of native CXCL15 in mouse lung extract based on a 500 µg/mL extract loadThe concentrations of CXCL15 were measured in duplicate and interpolated from the CXCL15 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL15 concentration was determined to be 325.81 pg/mL.
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Sandwich ELISA - Mouse CXCL15 ELISA Kit (ab234567)To learn more about the advantages of recombinant antibodies see here.
