Mouse CXCL15 ELISA Kit (ab193720)
Key features and details
- Sensitivity: 0.8 ng/ml
- Range: 0.8 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CXCL15 ELISA Kit
See all CXCL15 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.8 ng/ml -
Range
0.8 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 120.1 102% - 128% Plasma 134 118% - 150% Cell culture media 135.5 124% - 144% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s CXCL15 mouse ELISA Kit (ab193720) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CXCL15 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CXCL15 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CXCL15 present in the samples. The wells are washed and biotinylated anti-mouse CXCL15 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CXCL15 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent D 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Biotinylated mouse CXCL15 detection antibody (lyophilized) 2 vials mouse CXCL15 Standards (lyophilized) 2 vials Pre-coated mouse CXCL15 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Chemotactic for neutrophils. Involved in lung-specific neutrophil trafficking during normal and inflammatory conditions. -
Tissue specificity
Expression restricted to the lung, produced by bronchoepithelial cells and is released into the airways. Expressed at low levels in fetal lung. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 15
- Chemokine (C-X-C motif) ligand 15
- Cxcl15
see all -
Database links
- Entrez Gene: 20309 Mouse
- SwissProt: Q9WVL7 Mouse
- Unigene: 64326 Mouse
Images
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Standard Curve.
Typical Standard Curve using ab193720 CXCL15 Mouse ELISA Kit.
