Human Thrombin-Antithrombin Complex ELISA Kit (TAT) (ab108907)
Key features and details
- Sensitivity: 0.84 ng/ml
- Range: 0.84 ng/ml - 120 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Milk, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Thrombin-Antithrombin Complex ELISA Kit (TAT)
See all Thrombin-Antithrombin Complex kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5.6% Inter-assay Sample n Mean SD CV% Overall 9% -
Sample type
Cell culture supernatant, Milk, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.84 ng/ml -
Range
0.84 ng/ml - 120 ng/ml -
Recovery
95 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Thrombin-Antithrombin Complex (TAT) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin-Antithrombin Complex concentrations in plasma, milk and cell culture supernatants.
A Thrombin-Antithrombin Complex specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin-Antithrombin Complex specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin-Antithrombin Complex captured in plate.
Note: It is not recommended to perform spike recovery in plasma samples due to large amount of endogenous Anti-thrombin III (mg/ml range) which will saturate the capture antibody and interfere with the binding of the TAT standard
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Thrombin-Antithrombin Complex Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Sealing Tapes 3 units Stop Solution 1 x 11ml Thrombin-Antithrombin Complex Microplate (12 x 8 well strips) 1 unit Thrombin-Antithrombin Complex Standard 1 vial -
Research areas
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Relevance
Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. -
Cellular localization
Secreted -
Alternative names
- antithrombin III
- AT3
- AT3D
see all -
Database links
- Entrez Gene: 462 Human
- Entrez Gene: 2147 Human
- Omim: 107300 Human
- Omim: 176930 Human
- SwissProt: P01008 Human
- SwissProt: P00734 Human
Images
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Sandwich ELISA - Thrombin-Antithrombin Complex (TAT) Human ELISA Kit
TAT measured in biological fluids, with background signal subtracted (duplicates +/- SD).
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Typical Standard CurveRepresentative Standard Curve Using ab108907.