Canine CXCL1 ELISA Kit (GRO alpha) (ab273183)
Key features and details
- Sensitivity: 5.4 pg/ml
- Range: 5.47 pg/ml - 350 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine CXCL1 ELISA Kit (GRO alpha)
See all CXCL1/GRO alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
5.4 pg/ml -
Range
5.47 pg/ml - 350 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 134.8 121% - 145% Plasma 128.3 109% - 141% Cell culture media 128.8 108% - 149% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine CXCL1 ELISA Kit (GRO alpha) (ab273183) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine CXCL1 (GRO alpha) in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine CXCL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine CXCL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine CXCL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine CXCL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Canine CXCL1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine CXCL1 Antibody 2 vials Canine CXCL1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
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Images
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Canine CXCL1 ELISA Kit (GRO alpha) standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.