Human CXCL13 ELISA Kit (BCA-1) (ab269370)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CXCL13 ELISA Kit (BCA-1)
See all BCA1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 2.5% Inter-assay Sample n Mean SD CV% Supernatant 3 0.9% -
Sample type
Cell culture supernatant, Saliva, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 117 111% - 123% Saliva 87 78% - 97% Serum 90 88% - 94% Cell culture media 84 78% - 88% Hep Plasma 80 75% - 85% EDTA Plasma 91 88% - 94% Cit plasma 82 79% - 85% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human CXCL13 ELISA Kit (BCA-1) (ab269370) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL13 (BCA-1) protein in edta plasma, hep plasma, saliva, serum, cit plasma, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human CXCL13 (BCA-1) with 1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CXCL13 (C-X-C motif chemokine 13), also known as BCA-1(B-lymphocyte chemoattractant/B cell-attracting chemokine 1) is a member of the CXC chemokine superfamily. CXCL13 is produced by stromal cells from the liver, spleen, lymph nodes and the gut. It binds to the CXCR5 receptor in lymphoid follicles controlling the homing signal of B cells and subsets of T cells to the lymphoid nodes, compartmentalizing lymphocytes and antigen presenting cells within the follicles of lymphoid tissues. CXCL13 is also required for the embryonic development of most of lymph nodes and Peyer’s patches. CXCL13-/- as well as CXCR5-/- mice lack secondary lymphoid structures and show a disrupted splenic architecture.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human CXCL13 (BCA-1) Capture Antibody 1 x 600µl 10X Human CXCL13 (BCA-1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human CXCL13 (BCA-1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5. -
Tissue specificity
Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANGIE
- ANGIE2
- B cell attracting chemokine 1
see all -
Database links
- Entrez Gene: 10563 Human
- Omim: 605149 Human
- SwissProt: O43927 Human
- Unigene: 100431 Human
Images
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Example of human CXCL13 standard curve in Sample Diluent NS.
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human CXCL13 standard curve comparisonStandard Curve comparison between human CXCL13 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Human CXCL13 standard curve comparisonStandard Curve comparison between the original human MMP3 SimpleStep ELISA kit (ab179881) and current human MMP3 SimpleStep ELISA kit (ab269370).
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Example of human CXCL13 standard curve in Sample Diluent NS.The CXCL13 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CXCL13 in human THP-1 supernatant treated or untreated with Hu-IFN-r, LPS and PBMC cell culture supernatant treated with or without 1.5% PHA-M for 36 hours.The concentrations of CXCL13 were measured in duplicates, interpolated from the CXCL13 standard curves and corrected for sample dilution. Undiluted samples are as follows: THP-1 treated 25%, THP-1 untreated 100%, PBMC treated 50% and PBMC untreated 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 1,292 pg/mL in neat THP-1 treated supernatant, undetectable in untreated THP-1 supernatant, 337 pg/mL in treated neat PBMC supernatant and 25 pg/mL in untreated neat PBMC supernatant.
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Interpolated concentrations of native CXCL13 in human serum, plasma-citrate, plasma-EDTA and plasma-heparin samples.The concentrations of CXCL13 were measured in duplicates in neat samples and interpolated from the CXCL13 standard curves. The interpolated values are plotted (mean +/- SD, n=2). The mean CXCL13 concentration was determined to be 13 pg/mL in serum, 12.6 pg/mL in plasma-citrate, 13.1 pg/mL in plasma-EDTA and 10 pg/mL in plasma-heparin.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable CXCL13 concentration was determined to be 12.7 pg/mL with a range of undetectable to 18.3 pg/mL.
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Sandwich ELISA - Human CXCL13 ELISA Kit (BCA-1) (ab269370)To learn more about the advantages of recombinant antibodies see here.
