Horse MCP1 ELISA kit (ab272035)
Key features and details
- Sensitivity: 8.2 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse
Overview
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Product name
Horse MCP1 ELISA kit
See all MCP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich (quantitative) -
Sensitivity
8.2 pg/ml -
Range
8.19 - 2000 -
Recovery
Sample specific recovery Sample type Average % Range Serum 119.6 114% - 130% Plasma 110.7 92% - 128% Cell culture media 126.5 112% - 114% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Horse -
Product overview
Horse MCP1 ELISA Kit is designed for the quantitative determination of Horse MCP1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for MCP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MCP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse MCP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MCP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 900X HRP-Streptavidin Concentrate 1 x 200µl Anti-Horse MCP1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Horse MCP1 Detection Antibody 2 vials Horse MCP1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see all -
Database links
- Entrez Gene: 100034136 Horse
- SwissProt: Q9TTQ3 Horse
Images
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Horse MCP1 standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.
Horse MCP1 ELISA kit (ab272035) Standard curve.