Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 17, 2021

Anti-CXCL11 antibody [EPR21755-173] (ab216157)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21755-173] to CXCL11
Suitable for: WB, IP
Reacts with: Human

Product name

Anti-CXCL11 antibody [EPR21755-173]
See all CXCL11 primary antibodies
Description

Rabbit monoclonal [EPR21755-173] to CXCL11
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human CXCL11 recombinant protein (aa22-94); THP-1 treated with 200 ng/ml interferon-gamma (ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, followed by 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate. IP: THP-1 treated with 200 ng/ml interferon-gamma (ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, followed by 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21755-173
Isotype

IgG
Research areas

Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution + Human CXCL11 recombinant protein (aa 22-94) at 0.01 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 10 kDa
Observed band size: 9 kDa
why is the actual band size different from the predicted?

Exposure time: 15 seconds

Blocking/Dilution: 5% NFDM/TBST
Western blot - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

All lanes : Anti-CXCL11 antibody [EPR21755-173] (ab216157) at 1/1000 dilution

Lane 1 : THP-1 (hman monocytic leukemia cell line) whole cell lysate
Lane 2 : THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then 300 ng/ml Brefeldin A (BFA) was added to the treated cells for 20 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 10 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?

Exposure time: 37 seconds

Blocking/Dilution: 5% NFDM/TBST

The expression profile observed is consistent with what has been described in the literature (PMID: 17142784).
Immunoprecipitation - Anti-CXCL11 antibody [EPR21755-173] (ab216157)

CXCL11 was immunoprecipitated from 0.35mg of THP-1 (human monocytic leukemia cell line) whole cell lysate with ab216157 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216157 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5,000 dilution.

Lane 1: THP-1 (human monocytic leukemia cell line) treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate 10ug (Input).
Lane 2: ab216157 IP in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216157 in THP-1 treated with 200 ng/ml interferon-gamma (IFN-gamma, ab9659) and 50 ng/ml lipopolysaccharide (LPS) for 24 hours, then added 300 ng/ml Brefeldin A (BFA) for 20 hours, whole cell lysate.

Blocking/Dilution buffer: 5% NFDM/TBST
Anti-CXCL11 antibody [EPR21755-173] (ab216157)

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