Anti-CXCL11 antibody [EPR11076] (ab181035)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11076] to CXCL11
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-CXCL11 antibody [EPR11076]
See all CXCL11 primary antibodies -
Description
Rabbit monoclonal [EPR11076] to CXCL11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal spleen and THP1 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11076 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/1000 dilution (Purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates prepared in RIPA lysis method
Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates prepared in 1%SDS Hot lysis method
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 10 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?We recommend to use 1%SDS Hot lysis prepararation method.
For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).
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Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/5000 dilution (Purified) + Human fetal spleen lysates prepared in RIPA lysis method at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 10 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
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All lanes : Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/1000 dilution (Unpurified)
Lane 1 : Human spleen lysate
Lane 2 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 10 kDa
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