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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)

Anti-CXCL11 antibody [EPR11076] (ab181035)

Price and availability

278 083 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-CXCL11 antibody [EPR11076] (ab181035)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11076] to CXCL11
  • Suitable for: WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-CXCL11 antibody [EPR11076]
    See all CXCL11 primary antibodies
  • Description

    Rabbit monoclonal [EPR11076] to CXCL11
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal spleen and THP1 lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11076
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

Images

  • Western blot - Anti-CXCL11 antibody [EPR11076] (ab181035)
    Western blot - Anti-CXCL11 antibody [EPR11076] (ab181035)
    All lanes : Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/1000 dilution (Purified)

    Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates prepared in RIPA lysis method
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates prepared in 1%SDS Hot lysis method

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 10 kDa
    Observed band size: 14 kDa
    why is the actual band size different from the predicted?



    We recommend to use 1%SDS Hot lysis prepararation method.

    For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or here (downloadable copy).

  • Western blot - Anti-CXCL11 antibody [EPR11076] (ab181035)
    Western blot - Anti-CXCL11 antibody [EPR11076] (ab181035)
    Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/5000 dilution (Purified) + Human fetal spleen lysates prepared in RIPA lysis method at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 10 kDa
    Observed band size: 14 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-CXCL11 antibody [EPR11076] (ab181035)
    Western blot - Anti-CXCL11 antibody [EPR11076] (ab181035)
    All lanes : Anti-CXCL11 antibody [EPR11076] (ab181035) at 1/1000 dilution (Unpurified)

    Lane 1 : Human spleen lysate
    Lane 2 : THP1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP-labeled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 10 kDa

  • Anti-CXCL11 antibody [EPR11076] (ab181035)
    Anti-CXCL11 antibody [EPR11076] (ab181035)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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