Human MIP2 Matched Antibody Pair Kit (ab220122)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 3.1 pg/ml - 200 pg/ml
Overview
-
Product name
Human MIP2 Matched Antibody Pair Kit
See all CXCL2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.54 pg/ml -
Range
3.1 pg/ml - 200 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human MIP2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MIP2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab184862. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human MIP2 Capture Antibody 2 x 50µg 1 x 50µg Human MIP2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human MIP2 Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- C-X-C motif chemokine 2
- Chemokine (C X C motif) ligand 2
- Chemokine, CXC motif, ligand 2
see all -
Database links
- Entrez Gene: 2920 Human
- Omim: 139110 Human
- SwissProt: P19875 Human
- Unigene: 75765 Human
Images
-
Human MIP2 standard curve
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Human MIP2 Matched Antibody Pair Kit (ab220122)To learn more about the advantages of recombinant antibodies see here.
