Human MIP2 ELISA Kit (CXCL2) (ab184862)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 2.3 pg/ml - 150 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MIP2 Capture Antibody 1 x 600µl 10X Human MIP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human MIP2 Lyophilized Recombinant Protein (ab9842) 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 2
- Chemokine (C X C motif) ligand 2
- Chemokine, CXC motif, ligand 2
see all -
Database links
- Entrez Gene: 2920 Human
- Omim: 139110 Human
- SwissProt: P19875 Human
- Unigene: 75765 Human
Images
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Other - Human MIP2 ELISA Kit (CXCL2) (ab184862)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Linearity of dilution for biologicals.Interpolated values corrected by dilution factor (mean +/- SD) are graphed.
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Typical Standard CurveExample of MIP2 standard curve in Sample Diluent NS using ab184862
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Typical Standard CurveExample of MIP2 standard curve in Sample Diluent 25BP using ab184862
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Specificity of MIP2 on stimulated and non stimulated media supernatants.Human PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37˚C in the presence or absence of PHA. The concentrations of MIP2 were interpolated from the calibration curve and corrected for sample dilution. The mean MIP2 concentration was 67 pg/mL on unstimulated PBMC supernatants and 773 pg/mL on stimulated PBMCs supernatants.
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Levels of MIP2 in human serum samples.Human MIP2 levels in individual healthy donors. Ten individual healthy donors were evaluated for the presence of MIP2 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent 25BP and corrected for sample dilution (1:4). The mean level of Human MIP2 was found at 84.757 pg/mL with a range of 26.767 – 266.256 pg/mL.
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Sandwich ELISA - Human MIP2 ELISA Kit (CXCL2) (ab184862)To learn more about the advantages of recombinant antibodies see here.
