Human MIP2 ELISA Kit, Fluorescent (ab229427)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.26 pg/ml
- Range: 0.29 pg/ml - 300 pg/ml
- Sample type: EDTA Plasma, Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MIP2 ELISA Kit, Fluorescent
See all CXCL2 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Media 24 2.81% Inter-assay Sample n Mean SD CV% Media 24 3.46% -
Sample type
Serum, Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.26 pg/ml -
Range
0.29 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101.3 89.9% - 111.09% Plasma 100.07 87.61% - 116.98% Cell culture media 106.57 97.09% - 116.04% EDTA Plasma 96.92 94.06% - 100.01% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Goat, Cow, Pig -
Product overview
MIP2 (CXCL2) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of MIP2 (CXCL2) protein in human serum, plasma and cell culture supernatants.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
Macrophage inflammatory protein 2 (MIP2), otherwise known as CXCL2, GRO-beta, or Hematopoietic synergistic factor, is a 7.9 kDa heparin-binding chemokine that has potent effects in the response to inflammation and induction of peripheral tolerance. It is secreted by activated monocytes, neutrophils and inflamed mucosal epithelial cells in response to inflammatory stimuli such as IL-1β. MIP2 recruits granulocytic neutrophils and macrophages at sights of inflammation, and causes degranulation of these effector cells at the inflammatory site. It has also been hypothesized that MIP2 acts to synergize the effects of Granulocyte macrophage colony-stimulating factor (GM-CSF) and Macrophage colony-stimulating factor (M-CSF), leading to a larger recruitment of neutrophils and macrophages at the site of inflammation.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human MIP2 Capture Antibody 1 x 600µl 10X Human MIP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent 4BI 1 x 6ml Human MIP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
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Function
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsThe N-terminal processed form GRO-beta(5-73) is produced by proteolytic cleavage after secretion from bone marrow stromal cells. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 2
- Chemokine (C X C motif) ligand 2
- Chemokine, CXC motif, ligand 2
see all -
Database links
- Entrez Gene: 2920 Human
- Omim: 139110 Human
- SwissProt: P19875 Human
- Unigene: 75765 Human
Images
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Other - Human MIP2 ELISA Kit, Fluorescent (ab229427)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human MIP2 (CXCL2) standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilution for biologicalsSamples were prepared according to linearity of dilution section described in Typical Sample Values section of the protocol. Interpolated values corrected by dilution factor (mean +/- SD) are graphed.
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Specificity of MIP2 on stimulated and non stimulated media supernatantsHuman PBMCs were cultured in RPMI supplemented with 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL penicillin and 100 µg/mL streptomycin. Cells were cultured for 2 days at 37˚C in the presence or absence of PHA. The concentrations of MIP2 were interpolated from the calibration curve and corrected for sample dilution. The mean MIP2 concentration was 67 pg/mL on unstimulated PBMC supernatants and 773 pg/mL on stimulated PBMCs supernatants.
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MIP2 levels in individual healthy donorsTen individual healthy donors were evaluated for the presence of MIP2 in serum using this assay. Results were interpolated from the standard curve in Sample Diluent 25BP and corrected for sample dilution (1:4). The mean level of Human MIP2 was found at 84.757 pg/mL with a range of 26.767 – 266.256 pg/mL.
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Sandwich ELISA - Human MIP2 ELISA Kit, Fluorescent (ab229427)To learn more about the advantages of recombinant antibodies see here.
