Anti-IRAK-1 antibody (ab245342)
Key features and details
- Rabbit polyclonal to IRAK-1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-IRAK-1 antibody
See all IRAK-1 primary antibodies -
Description
Rabbit polyclonal to IRAK-1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide within Human IRAK-1 aa 575-625. The exact sequence is proprietary. (NP_001560.2).
Database link: P51617 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245342 was affinity purified using an epitope specific to IRAK-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IRAK-1 antibody (ab245342)All lanes : Anti-IRAK-1 antibody (ab245342) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 30 seconds
-
Immunoprecipitation - Anti-IRAK-1 antibody (ab245342)IRAK-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245342 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245342 at 1 µg/ml.
Lane 1: ab245342 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
