Anti-IRAK-1 antibody (ab238)
Key features and details
- Rabbit polyclonal to IRAK-1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IRAK-1 antibody
See all IRAK-1 primary antibodies -
Description
Rabbit polyclonal to IRAK-1 -
Host species
Rabbit -
Specificity
At least four isoforms of IRAK are known to exist; this antibody will detect all four isoforms. IRAK antibody is predicted to not cross-react with other members of the IRAK protein family.
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Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseRat
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Immunogen
Synthetic peptide corresponding to Human IRAK-1 aa 700-712 (C terminal).
(Peptide available asab6230) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Affinity chromatography purified via peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-IRAK-1 antibody (ab238)All lanes : Anti-IRAK-1 antibody (ab238) at 1 µg/ml
Lane 1 : L1210 cell lysate
Lane 2 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa1h incubation at RT in 5% NFDM/TBST.
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody (ab238)ab238 at 10µg/ml staining Hela cells by ICC/IF -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK-1 antibody (ab238)IHC image of ab238 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab238, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody (ab238) Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab238 staining IRAK-1 in murine RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton-X100 in 2% BSA for 15 minutes, blocked with 2% BSA for 1 hour at 22°C and then incubated with ab238 at a 1/150 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated chicken anti-rabbit IgG (H+L) used at a 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-IRAK-1 antibody (ab238)Immunofluorescence of IRAK-1 in HeLa cells using ab238 at 20 ug/ml.
