JunD Transcription Factor Assay Kit (Colorimetric) (ab207198)
Key features and details
- Assay type: Semi-quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 3 hr 30 min
- Sample type: Nuclear Extracts
- Sensitivity: 1250 ng/well
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 5 x 96 tests 10X Antibody Binding Buffer 1 x 2.2ml 1 x 11ml 10X Wash Buffer 1 x 22ml 1 x 110ml 96-well assay plate 1 unit 5 units Anti-rabbit HRP-conjugated IgG (0.25 μg/μL) 1 x 11µl 1 x 55µl AP-1 Mutated oligonucleotide (10 pmol/μL) 1 x 100µl 1 x 500µl AP-1 Wild-type oligonucleotide (10 pmol/μL) 1 x 100µl 1 x 500µl Binding Buffer 1 x 10ml 1 x 50ml Developing Solution 1 x 11ml 1 x 55ml Dithiothreitol (DTT) (1 M) 1 x 100µl 1 x 500µl JunD antibodies 1 x 11µl 1 x 55µl K-562(TPA) nuclear extract (2.5µg/μL) 1 x 40µl 1 x 200µl Lysis Buffer 1 x 10ml 1 x 50ml Plate sealer 1 unit 5 units Poly [d(l-c)] (17 µg/μL) 1 x 100µl 1 x 500µl Protease Inhibitor Cocktail 1 x 100µl 1 x 500µl Stop Solution 1 x 11ml 1 x 55ml -
Research areas
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Function
Transcription factor binding AP-1 sites. -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP domain. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- Activator protein 1
- AP 1
- AP1
see all -
Database links
- Entrez Gene: 3727 Human
- Entrez Gene: 16478 Mouse
- Entrez Gene: 24518 Rat
- Omim: 165162 Human
- SwissProt: P17535 Human
- SwissProt: P15066 Mouse
- SwissProt: P52909 Rat
- Unigene: 2780 Human
see all
Images
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Nuclear extracts from K-562 cells stimulated with TPA were assayed for activity of AP1 family member Jun D.
Nuclear extracts from K-562 cells stimulated with TPA (Grey) were assayed for activity of AP1 family member Jun D with 5 µg/well of nuclear extract in the absence or presence of wild-type (Black) or mutated (White) consensus binding oligonucleotides. These results are provided for demonstration purposes only.
