Anti-SERPINB1/PI2 antibody (ab47731)
Key features and details
- Rabbit polyclonal to SERPINB1/PI2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SERPINB1/PI2 antibody
See all SERPINB1/PI2 primary antibodies -
Description
Rabbit polyclonal to SERPINB1/PI2 -
Host species
Rabbit -
Specificity
This antibody is specific for SERPINB1/PI2.
-
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
Immunogen
Synthetic peptide corresponding to Human SERPINB1/PI2 (N terminal).
-
General notes
This product was previously labelled as SERPINB1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-SERPINB1/PI2 antibody (ab47731)All lanes : Anti-SERPINB1/PI2 antibody (ab47731) at 1/1000 dilution
All lanes : MH-S cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size: 43 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible degradation product)
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERPINB1/PI2 antibody (ab47731)ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
