Human CXCL9 ELISA Kit, Fluorescent (ab270891)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2.6 pg/ml
- Range: 2.93 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Extracts
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human CXCL9 ELISA Kit, Fluorescent
See all CXCL9 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 3 3.4% Inter-assay Sample n Mean SD CV% Supernatant 5 4.9% -
Sample type
Cell culture supernatant, Serum, Tissue Extracts, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2.6 pg/ml -
Range
2.93 pg/ml - 3000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 90 86% - 92% Serum 115 103% - 131% Tissue Extracts 109 104% - 116% EDTA Plasma 109 99% - 125% Cit plasma 119 110% - 127% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
CXCL9 in vitro CatchPoint® SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of CXCL9 protein in human serum, plasma cell culture supernatants, and tissue extract samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.orgThe CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
CXCL9 is a small cytokine belonging to the CXC chemokine subfamily that lacks an ELR motif in front of the first cysteine. CXCL9, also known as MIG (Monokine Induced by Gamma Interferon) is a T-cell chemoattractant, which is induced by Interferon Gamma, This subfamily also includes Interferon Gamma Induced Protein 10 (IP-10 or CXCL10) and Interferon Inducible T-cell Alpha Chemoattractant (I-TAC or CXCL11) whose genes are located near the gene for CXCL9 on human chromosome 4, CXCL9, IP-10, and I-TAC all elicit their chemotactic functions by interacting with the G protein coupled chemokine receptor CXCR3 (GPR9 or CD183).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human CXCL9 Capture Antibody 1 x 600µl 10X Human CXCL9 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human CXCL9 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
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Function
Cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response. Chemotactic for activated T-cells. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 9
- chemokine (C-X-C motif) ligand 9
- CMK
see all -
Database links
- Entrez Gene: 4283 Human
- Omim: 601704 Human
- SwissProt: Q07325 Human
- Unigene: 77367 Human
Images
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Catchpoint ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human CXCL9 standard curve in Sample Diluent NS.The CXCL9 standard curve was prepared as described in Section 10. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of recombinant human CXCL9 protein spiked into human serum and plasma samples.The concentrations of CXCL9 were measured in duplicates, interpolated from the CXCL9 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% (neat), plasma (citrate) 100% (neat), and plasma (EDTA) 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native CXCL9 in PHA-M stimulated and unstimulated human PBMC cell culture supernatant (2 days) samples.The concentrations of CXCL9 were measured in duplicates, interpolated from the CXCL9 standard curves and corrected for sample dilution. Undiluted samples are as follows: PHA-M stimulated PBMC supernatant 2.5% and unstimulated PBMC supernatant 100% (neat). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 20.4 ng/mL in neat PHA-M stimulated PBMC supernatant and 1.62 ng/mL in neat unstimulated PBMC supernatant.
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Interpolated concentrations of native CXCL9 in PHA-M stimulated human PBMC cell extract based on a 200 µg/mL extract loadThe concentrations of CXCL9 were measured in duplicate and interpolated from the CXCL9 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL9 concentration was determined to be 993.9 pg/mL in PHA-M stimulated PBMC cell extract and 824.9 pg/mL in thyroid tissue extract.
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Serum from ten individual healthy female human donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean of detectable CXCL9 concentration was determined to be 90.2 pg/mL with a range of 47.8 – 149 pg/mL. Detectable concentrations are defined as concentration above the lowest standard concentration.
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Comparison of CXCL9 in unstimulated and PHA-M stimulated human PBMC cell supernatants.Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 2 days. The concentrations of CXCL9 were measured in three different dilutions of the supernatant samples in duplicates and interpolated from the CXCL9 standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean CXCL9 concentration was determined to be 21.3 ng/mL in PHA-M stimulated PBMC cell supernatant, 1.6 ng/mL in unstimulated supernatants and undetectable in media (not shown).
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Sandwich ELISA - Human CXCL9 ELISA Kit, Fluorescent (ab270891)To learn more about the advantages of recombinant antibodies see here.
