Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-CXCL5 + CXCL6 antibody [EPR22310-196]
    See all CXCL5 + CXCL6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: His-GST-tagged human CXCL5 recombinant protein (aa37-114); Human CXCL6 recombinant protein (aa40-114) without tag; A549 cells starved overnight, then treated with 10 ng/ml TNF-a, 10nM Phorbol-12-myristate-13-acetate (PMA) and 0.1% BSA for 24 hours, whole cell lysate. ICC/IF: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h). Flow Cyt: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22310-196

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)
    Flow Cytometry - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) cell line that was serum starved for 4h, then treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) (Red) / Untreated control (Green) labeling CXCL5 + CXCL6 with ab243097 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID: 9057843; 23922745).

  • Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)
    Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling CXCL5 + CXCL6 with ab243097 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) is observed. Tubulin was stained using the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) at 1/200 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA treatment. (PMID: 9057843; 23922745).

  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)
    Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)
    All lanes : Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097) at 1/500 dilution

    Lane 1 : A549 (human lung carcinoma cell line) starved overnight, whole cell lysate
    Lane 2 : A549 was starved overnight, then treated with 10ng/ml TNF-a, 10nM Phorbol-12-myristate-13-acetate (PMA) and 0.1% BSA for 24 hours, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 12 kDa


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID: 23922745).

  • Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)
    Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)
    All lanes : Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097) at 1/1000 dilution

    Lane 1 : His-GST-tagged human CXCL5 recombinant protein (aa37-114) 10 ng
    Lane 2 : Human CXCL6 recombinant protein (aa40-114) without tag 10 ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 12 kDa


    Exposure time: 3 seconds


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  • Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)
    Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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