Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Wednesday March 10, 2021

Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6
Suitable for: ICC/IF, Flow Cyt, WB
Reacts with: Human

Product name

Anti-CXCL5 + CXCL6 antibody [EPR22310-196]
See all CXCL5 + CXCL6 primary antibodies
Description

Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: His-GST-tagged human CXCL5 recombinant protein (aa37-114); Human CXCL6 recombinant protein (aa40-114) without tag; A549 cells starved overnight, then treated with 10 ng/ml TNF-a, 10nM Phorbol-12-myristate-13-acetate (PMA) and 0.1% BSA for 24 hours, whole cell lysate. ICC/IF: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h). Flow Cyt: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22310-196
Isotype

IgG
Research areas

Flow Cytometry - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) cell line that was serum starved for 4h, then treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) (Red) / Untreated control (Green) labeling CXCL5 + CXCL6 with ab243097 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.

The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID: 9057843; 23922745).
Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling CXCL5 + CXCL6 with ab243097 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) is observed. Tubulin was stained using the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594, ab195889) at 1/200 dilution (Red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA treatment. (PMID: 9057843; 23922745).
Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

All lanes : Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097) at 1/500 dilution

Lane 1 : A549 (human lung carcinoma cell line) starved overnight, whole cell lysate
Lane 2 : A549 was starved overnight, then treated with 10ng/ml TNF-a, 10nM Phorbol-12-myristate-13-acetate (PMA) and 0.1% BSA for 24 hours, whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 12 kDa

Exposure time: 3 minutes

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID 9057843; PMID: 23922745).
Western blot - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

All lanes : Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097) at 1/1000 dilution

Lane 1 : His-GST-tagged human CXCL5 recombinant protein (aa37-114) 10 ng
Lane 2 : Human CXCL6 recombinant protein (aa40-114) without tag 10 ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 12 kDa

Exposure time: 3 seconds

Blocking/Diluting buffer and concentration: 5% NFDM/TBST.
Anti-CXCL5 + CXCL6 antibody [EPR22310-196] (ab243097)

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