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Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6 - BSA and Azide free
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free
    See all CXCL5 + CXCL6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h). Flow Cyt: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h).

  • General notes

    Ab243559 is the carrier-free version of ab243097. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab243559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Flow Cytometry - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)
    Flow Cytometry - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) cell line that was serum starved for 4h, then treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) (Red) / Untreated control (Green) labeling CXCL5 + CXCL6 with ab243097 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID: 9057843; 23922745).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243097).

  • Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)
    Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling CXCL5 + CXCL6 with ab243097 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) is observed. Tubulin was stained using the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) at 1/200 dilution (Red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

    The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA treatment. (PMID: 9057843; 23922745).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243097).

  • Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)
    Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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