Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)
![Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)](https://www.abcam.com/ps/products/243/ab243559/Images/ab243559-333584-anti-cxcl5-cxcl6-antibody-epr22310-196-flow-cytometry.jpg "Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6 - BSA and Azide free
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free
See all CXCL5 + CXCL6 primary antibodies -
Description
Rabbit monoclonal [EPR22310-196] to CXCL5 + CXCL6 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h). Flow Cyt: A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h).
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General notes
Ab243559 is the carrier-free version of ab243097. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab243559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22310-196 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A549 (human lung carcinoma cell line) cell line that was serum starved for 4h, then treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) (Red) / Untreated control (Green) labeling CXCL5 + CXCL6 with ab243097 at 1/500 dilution, compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA. (PMID: 9057843; 23922745).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243097).
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![Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)](https://www.abcam.com/ps/products/243/ab243559/Images/ab243559-333583-anti-cxcl5-cxcl6-antibody-epr22310-196-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling CXCL5 + CXCL6 with ab243097 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A549 cells treated with TNF alpha (10ng/ml 24h), PMA (10nM 24h) and BSA (0.1% 24h) is observed. Tubulin was stained using the Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, ab195889) at 1/200 dilution (Red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
The expression of CXCL5 and CXCL6 is induced by TNF-a and PMA treatment. (PMID: 9057843; 23922745).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243097).
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![Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)](https://www.abcam.com/ps/products/243/ab243559/Images/ab243559-5-benefits-of-recombinant-antibodies.png)
Anti-CXCL5 + CXCL6 antibody [EPR22310-196] - BSA and Azide free (ab243559)
