Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505)
Key features and details
- Rabbit monoclonal [EP13083] to CXCL5 + CXCL6
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CXCL5 + CXCL6 antibody [EP13083]
See all CXCL5 + CXCL6 primary antibodies -
Description
Rabbit monoclonal [EP13083] to CXCL5 + CXCL6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549 cell lysate; Human CLCX5 recombinant protein (aa 5-78) and Human CLCX6 recombinant protein (GST-Tag) (aa 37-114); Human colonic carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP13083 -
Isotype
IgG -
Research areas
Images
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Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/5000 dilution + A549 (Human lung carcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 12 kDa
Exposure time: 3 minutes5% NFDM/TBST: Blocking and diluting buffer.
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Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling CXCL5 + CXCL6 with ab198505 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin.
Inset image: negative control obtained using PBS instead of ab198505 and secondary antibody only.
Note: Cytoplasm staining on human colonic carcinoma tissue was observed.Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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CXCL5 + CXCL6 was immunoprecipitated from 1mg of A549 (Human lung carcinoma cell line) whole cell extract with ab198505 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab198505 at 1/1000 dilution (lanes 1 and 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Input A549 whole cell extract (10µg).
Lane 2: A549 whole cell extract.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198505 in A549 (Human lung carcinoma) whole cell extract
Blocking and dilution buffer and concentration: 5% NFDM/TBST. -
Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/5000 dilution + Human CLCX5 recombinant protein: aa 5-78 at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Exposure time: 30 seconds5% NFDM/TBST: Blocking and diluting buffer.
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Anti-CXCL5 + CXCL6 antibody [EP13083] (ab198505) at 1/1000 dilution + Human CLCX6 recombinant protein (GST-Tag): aa 37-114 at 0.01 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Exposure time: 5 seconds5% NFDM/TBST: Blocking and diluting buffer.