Monkey MCP1 ELISA Kit (ab242231)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 16 pg/ml
- Range: 9.4 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Monkey, Cynomolgus monkey, Rhesus monkey
Overview
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Product name
Monkey MCP1 ELISA Kit
See all MCP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 3.8% Inter-assay Sample n Mean SD CV% serum 3 2.7% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
16 pg/ml -
Range
9.4 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 98 91% - 102% Serum 110 96% - 118% Cell culture media 80 76% - 84% Hep Plasma 112 98% - 118% EDTA Plasma 107 101% - 116% Cit plasma 93 91% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Monkey, Cynomolgus monkey, Rhesus monkey -
Product overview
Monkey MCP1 ELISA Kit (ab242231) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Monkey MCP1 with 2.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Monkey MCP1 Capture Antibody 1 x 600µl 10X Monkey MCP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Monkey MCP1 Lyophilized recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsProcessing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 2
- CCL2
- CCL2_HUMAN
see all
Images
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Other - Monkey MCP1 ELISA Kit (ab242231)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of monkey MCP1 standard curve in Sample Diluent NS.The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Example of monkey MCP1 standard curve in Sample Diluent 50BS.The MCP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native MCP1 in rhesus macaque serum and plasma samples.The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 496.0 pg/mL in neat serum, 157.9 pg/mL in plasma (citrate), 198.0 pg/mL in neat plasma (heparin), and 169.7 pg/mL in neat plasma (EDTA). -
Interpolated concentrations of native MCP1 in stimulated rhesus macaque peripheral blood monocyte cell (PBMC) supernatant samples.Rhesus macaque PBMCs were cultured in the presence or absence of 50 ng/mL PMA and 1 µg/mL Ionomycin for 24 hours. The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MCP1 concentration was determined to be 240.3 pg/mL in neat stimulated PBMC cell culture supernatant and undetectable in unstimulated PBMC supernatant and naive cell culture media (not shown).
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Comparison of rhesus macaque and cynomolgus monkey serum, plasma, and PMA + Ionomycin stimulated and unstimulated PBMC cell culture supernatants (SN) (24 hours).The concentrations of MCP1 were measured in duplicates, interpolated from the MCP1 standard curves and corrected for sample dilution. The interpolated dilution factor corrected values in neat samples are plotted (mean +/- SD, n=2). -
Sandwich ELISA - Monkey MCP1 ELISA Kit (ab242231)To learn more about the advantages of recombinant antibodies see here.
