Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)
![Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)](https://www.abcam.com/ps/products/222/ab222100/Images/ab222100-353427-anti-kir3dl1-kir3ds1-antibody-epr22876-121-western-blot-human.jpg "Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] -
Description
Rabbit monoclonal [EPR22876-121] to KIR3DL1 + KIR3DS1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB HumanRecombinant fragment
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag, whole cell lysate, HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag, whole cell lysate; IHC-P: Human endometrium and spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22876-121 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 2 : HEK-293T transfected with KIR3DL1 expression vector containing a myc-His-tag®, whole cell lysate
Lanes 3 & 5 : HEK-293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate
Lane 4 : HEK-293T transfected with KIR3DL2 expression vector containing a myc-His-tag®, whole cell lysate
Lane 6 : HEK-293T transfected with KIR3DS1 expression vector containing a myc-His-tag®, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 60,60-80 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 and KIR3DS1 are glycoproteins. The molecular weight observed are consistent with what have been described in the literature (PMID:17202323, PMID:16423035).
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![Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)](https://www.abcam.com/ps/products/222/ab222100/Images/ab222100-353426-anti-kir3dl1-kir3ds1-antibody-epr22876-121-western-blot-nk-92-human.jpg)
Western blot - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)All lanes : Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100) at 1/1000 dilution
Lane 1 : Untreated NK-92 (human malignant non-hodgkins lymphoma natural killer cell) whole cell lysate
Lane 2 : NK-92 treated with 2uM 5-aza-2-deoxycytidine for 48 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 40-70 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and Dilution Buffer and concentration: 5% NFDM/TBST. KIR3DL1 is a glycoprotein, its expression is induced after 5-aza-2-deoxycytidine treatment. The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:12370356, PMID:16423035).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)](https://www.abcam.com/ps/products/222/ab222100/Images/ab222100-3-anti-kir3dl1-kir3ds1-antibody-epr22876-121-ihcp-nk-t-lymphoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)Immunohistochemical analysis of paraffin-embedded human NK-T lymphoma tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human NK-T lymphoma (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)](https://www.abcam.com/ps/products/222/ab222100/Images/ab222100-2-anti-kir3dl1-kir3ds1-antibody-epr22876-121-ihcp-spleen-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in some lymphocytes of human spleen (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)](https://www.abcam.com/ps/products/222/ab222100/Images/ab222100-1-anti-kir3dl1-kir3ds1-antibody-epr22876-121-ihcp-endometrium-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling KIR3DL1+KIR3DS1 with ab222100 at 1/500 dilution (1.438 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in some stromal cells of human endometrium (PMID: 22140359) is observed. The section was incubated with ab222100 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)](https://www.abcam.com/ps/products/222/ab222100/Images/ab222100-8-benefits-of-recombinant-antibodies.png)
Anti-KIR3DL1 + KIR3DS1 antibody [EPR22876-121] (ab222100)