Rat CXCL10 ELISA Kit (ab270896)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 87.45 pg/ml
- Range: 117.18 pg/ml - 7500 pg/ml
- Sample type: Cell culture supernatant
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CXCL10 ELISA Kit
See all IP10 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 4.1% Inter-assay Sample n Mean SD CV% Supernatant 3 6.7% -
Sample type
Cell culture supernatant -
Assay type
Sandwich (quantitative) -
Sensitivity
87.45 pg/ml -
Range
117.18 pg/ml - 7500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 96 85% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Rat -
Product overview
Rat CXCL10 ELISA kit (ab270896) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL10 protein in rat cell and tissue supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate rat CXCL10 with 87.45 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Rat C-X-C motif chemokine 10 (CXCL10) is a 10 kDa protein that is secreted by several cell types in response to interferon-gamma. These cell types include monocytes, endothelial cells and fibroblasts. Upon secretion, CXCL10 is cleaved into an 8.7kD biologically active protein to function in chemotaxis for T-cells, NK cells, monocytes/macrophages and dendritic cells. In addition, CXCL10 has antitumor activity through the inhibition of bone marrow colony formation and angiogenesis. CXCL10 elicits its effects by binding to the cell surface chemokine receptor 3 (CXCR3). Rat CXCL10 shares a 79% and 68% sequence identity with mouse and human, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat CXCL10 Capture Antibody 1 x 600µl 10X Rat CXCL10 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Plate Seals 1 unit Rat CXCL10 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Chemotactic for monocytes and T-lymphocytes. Binds to CXCR3. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interferon gamma induced factor MOB1, mouse, homolog of
- Interferon gamma induced protein 10
- 10 kDa interferon gamma induced protein
see all -
Database links
- Entrez Gene: 245920 Rat
- SwissProt: P48973 Rat
- Unigene: 10584 Rat
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of rat CXCL10 standard curve in Sample Diluent NS.The CXCL10 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native CXCL10 in rat tissue culture supernatant samples.The concentrations of CXCL10 were measured in duplicates, interpolated from the CXCL10 standard curves and corrected for sample dilution. Undiluted samples are as follows: 5 ug/mL LPS stimulated spleen culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL10 concentration was determined to be 6364.20 pg/mL in and stimulated spleen supernatant.
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Sandwich ELISA - Rat CXCL10 ELISA Kit (ab270896)To learn more about the advantages of recombinant antibodies see here.
