Rat CXCL1 ELISA Kit (GRO alpha) (ab219044)
Key features and details
- Sensitivity: 1.61 pg/ml
- Range: 9.38 pg/ml - 600 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat
Overview
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Product name
Rat CXCL1 ELISA Kit (GRO alpha)
See all CXCL1/GRO alpha kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Rat serum 8 10.1% Inter-assay Sample n Mean SD CV% Rat serum 3 10% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.61 pg/ml -
Range
9.38 pg/ml - 600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99 88% - 109% Cell culture extracts 103 100% - 104% Cell culture media 95 90% - 102% Tissue Culture Media 96 85% - 116% Hep Plasma 114 110% - 118% EDTA Plasma 106 103% - 108% Cit plasma 97 88% - 112% -
Assay time
1h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat
Does not react with: Cow -
Product overview
Rat CXCL1 ELISA Kit (GRO alpha) (ab219044) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of CXCL1 (GRO alpha) protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat CXCL1 with 1.61 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
CXCL1 (previously called GRO1 oncogene) is a member of the CXC family of chemokines. CXCL1 expression is reported in lymphocytes such as macrophages and neutrophils as well as epithelial cells. It plays a role in neutrophil chemotaxis. CXCL1 is involved in spinal cord development via inhibition of oligodendrocyte precursor migration as well angiogenesis, arteriogenesis, inflammation, wound healing and tumorigenesis.
Rat CXCL2 and CXCL3 share 65% and 64% sequence homology with rat CXCL1, respectively. Additionally, mouse CXCL1 is 89% homologous with rat CXCL1.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Rat CXCL1 Capture Antibody 1 x 600µl 10X Rat CXCL1 Detector Antibody 1 x 600µl 10X Wash Buffer PT 2 x 30ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 2 x 6ml Plate Seals 1 unit Rat CXCL1 Lyophilized Recombinant Protein 2 vials Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
Database links
- Entrez Gene: 81503 Rat
- SwissProt: P14095 Rat
- Unigene: 10907 Rat
Images
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Example of rat CXCL1 (GRO alpha) standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
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Example of rat CXCL1 (GRO alpha) standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Rat CXCL1 (GRO alpha) standard curve comparison dataStandard curve comparison between rat CXCL1 SimpleStep ELISA® kit (GRO alpha) and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows comparable sensitivity.
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Interpolated concentrations of spiked CXCL1 (GRO alpha) in rat serum and plasma samples.The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 930.66 pg/mL in neat serum, 756.22 pg/mL in neat plasma (citrate), 674.04 pg/mL in neat plasma (EDTA), and 891.86 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native CXCL1 (GRO alpha) in rat serum and plasma samples.The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 245.16 pg/mL in neat serum, 75.79 pg/mL in neat plasma (citrate), 24.53 pg/mL in neat plasma (EDTA), and 137.19 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native CXCL1 (GRO alpha) in rat tissue culture supernatant samples.The concentrations of CXCL1 (GRO alpha) were measured in duplicates, interpolated from the CXCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: rat lung supernatant 1% and rat spleen supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 57,709.68 pg/mL in neat rat lung supernatant and 822.66 pg/mL in neat rat spleen supernatant. The rat lung supernatant was cultured for six days in RPMI base media with 10% Fetal Bovine Serum (FBS) plus 5 µg/mL of LPS. The rat spleen supernatant was cultured for three days in RPMI base media with 10% FBS.
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Interpolated concentrations of native CXCL1 (GRO alpha) in rat C6 cell extract based on a 500 µg/mL extract load.The concentrations of CXCL1 (GRO alpha) were measured in duplicate and interpolated from the CXCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean CXCL1 concentration was determined to be 532.46 pg/mL in C6 cell extract.
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Sandwich ELISA - Rat CXCL1 ELISA Kit (GRO alpha) (ab219044)To learn more about the advantages of recombinant antibodies see here.
