Rat CXCL1 Matched Antibody Pair Kit (GRO alpha) (ab219543)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Rat
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Rat CXCL1 Matched Antibody Pair Kit (GRO alpha)
See all CXCL1/GRO alpha kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.7 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Rat -
Product overview
Rat CXCL1 Matched Antibody Pair Kits (GRO alpha) include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant rat CXCL1 (GRO alpha).
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219044. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Rat CXCL1 Capture Antibody 2 x 50µg 1 x 50µg Rat CXCL1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Rat CXCL1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. -
Sequence similarities
Belongs to the intercrine alpha (chemokine CxC) family. -
Post-translational
modificationsN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-X-C motif chemokine 1
- chemokine (C-X-C motif) ligand 1
- Chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha)
see all -
Database links
- Entrez Gene: 81503 Rat
- SwissProt: P14095 Rat
- Unigene: 10907 Rat
Images
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Rat CXCL1 (GRO alpha) Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Rat CXCL1 Matched Antibody Pair Kit (GRO alpha) (ab219543)To learn more about the advantages of recombinant antibodies see here.
