Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Thyroglobulin antibody [EPR9730] (ab156008)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9730] to Thyroglobulin
  • Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Thyroglobulin antibody [EPR9730]
    See all Thyroglobulin primary antibodies

  • Description

    Rabbit monoclonal [EPR9730] to Thyroglobulin

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Thyroglobulin aa 1000-1100. The exact sequence is proprietary.
    Database link: P01266

  • Positive control

    • Mouse thyroid, rat thyroid, and Human thyroid lysates, Human thyroid gland follicular carcinoma tissue and Human thyroid gland papillary carcinoma tissue, TT cells

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR9730

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution (purified) + Rat thyroid lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 305 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] (ab156008)

    Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1:70 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (ab156008)

    Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1:500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    All lanes : Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution (purified)

    Lane 1 : Mouse thyroid lysates
    Lane 2 : ICR thyroid lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 305 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution (purified) + Human thyroid lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 305 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Flow Cytometry - Anti-Thyroglobulin antibody [EPR9730] (ab156008) This image is courtesy of an Abreview submitted by Sanjay Gawade

    Unpurified ab156008 staining Thyroglobulin in mouse thyroid cells by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy: Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.

    See Abreview

  • Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Western blot - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    All lanes : Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution (unpurified)

    Lane 1 : Mouse thyroid lysate
    Lane 2 : Rat thyroid lysate
    Lane 3 : Human thyroid lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 305 kDa
    Observed band size: 294-300 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroglobulin antibody [EPR9730] (ab156008)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.

  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Immunocytochemistry/ Immunofluorescence - Anti-Thyroglobulin antibody [EPR9730] (ab156008)

    Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.

  • Anti-Thyroglobulin antibody [EPR9730] (ab156008)
    Anti-Thyroglobulin antibody [EPR9730] (ab156008)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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