Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B] (ab136926)
Key features and details
- Rabbit monoclonal [G15-B] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B]
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies -
Description
Rabbit monoclonal [G15-B] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). Including the conserved pT-E-pY motif
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Positive control
- PC12 cell lysates (treated with EGF, PMA or EGF); HEK293 cells treated with hydrogen peroxide.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 8.00
Preservative: 0.05% Sodium azide
Constituents: 0.32% Tris HCl, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This immunoglobulin is the product of one single B-cell line from the crude anti-peptide polyclonal anti-serum. This antibody is purified using a proprietary technique and offers a completely post-translationally modified and properly glycosylated antibody. This offers increased stability. -
Clonality
Monoclonal -
Clone number
G15-B -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [G15-B] (ab136926) at 1/5000 dilution
Lane 1 : PC12 cell lysate - untreated
Lane 2 : PC12 cell lysate - treated with EGF-100ng/ml (5min) in serum-free DMEM
Lane 3 : PC12 cell lysate - treated with PMA-100nM (30min) in serum-free DMEM
Lane 4 : PC12 cell lysate treated with EGF-100ng/ml (5min) with 10% FBS in DMEM
Lysates/proteins at 50 µg per lane.
Predicted band size: 41, 43 kDa
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Immunocytochemical analysis of HEK293 cells labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.
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Immunocytochemical analysis of HEK293 cells (hydrogen peroxide treated) labelling phospho-ERK1 + ERK2 with ab136926 at 1/100 dilution.