Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E337] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free
    See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies

  • Description

    Rabbit monoclonal [E337] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody detects ERK1 phosphorylated on Threonine 202 and Tyrosine 204 and ERK2 phosphorylated on Threonine 185 and Tyrosine 187.

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human thyroid gland cancer tissue.

  • General notes

    ab232017 is the carrier-free version of ab32538 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab232017 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)
    Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)

    Immunocytochemistry/Immunofluorescence analysis of 4% paraformaldehyde A431+-EGF(100ng/ml,5min) labelling Erk1 (pT202/pY204) + Erk2 (pT185/pY187) with ab32538 at dilution of 1/200. The secondary antibody used was Alexa Fluor® 488 Goat-Anti-Rabbit IgG (ab150077) at dilution of 1/400. The counter stain was done with DAPI (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32538).

  • Flow Cytometry - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)
    Flow Cytometry - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)

    Overlay histogram showing HeLa cells stained with ab32538 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32538, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32538).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)

    Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer using anti-ERK1(pT202/pY204)/ERK2(pT185/pY187) (ab32538) at dilution of 1:50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32538).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)
    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [E337] - BSA and Azide free (ab232017)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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