Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab128159)
Key features and details
- Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies -
Description
Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
-
Immunogen
Synthetic peptide, corresponding to amino acids surrounding phosphorylated threonine 202 and tyrosine 204 of Human ERK1 (NP_002737.2) and phosphorylated threonine 185 and tyrosine 187 of Human ERK2 (NP_620407.1).
-
Positive control
- HeLa whole cell lysate, mock treated or treated with TPA
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab128159)All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab128159) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate, mock treated
Lane 2 : HeLa whole cell lysate, TPA treated
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 41, 43 kDa
Exposure time: 30 seconds
-
Immunoprecipitation - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab128159)ab128159 at 1 µg/ml staining ERK1 + ERK2 (phospho T202 + Y204 + T185 + Y187) in HeLa whole cell lysate immunoprecipitated using a Rabbit anti-ERK1 antibody (1 mg/IP; 20% of IP loaded/lane).
Lane 1: mock treated.
Lanes 2 and 3: TPA treated.
Lane 3: normal goat IgG.
Predicted band size: 41 kDa.
Exposure time: 30 seconds.
