Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

50 µl 335 040 ₸

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Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)

Key features and details

Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
Suitable for: ICC/IF, WB
Reacts with: Rat, Saccharomyces cerevisiae, Drosophila melanogaster
Isotype: IgG1

Product name

Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT]
See all Erk1 (pT202/pY204) + Erk2 (pT185/pY187) primary antibodies
Description

Mouse monoclonal [MAPK-YT] to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
Host species

Mouse
Tested Applications & Species

Application Species
ICC/IF
Rat

Drosophila melanogaster

WB
Rat

See all applications and species data
Immunogen

Synthetic peptide corresponding to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic peptide corresponds to the phosphorylated form of human ERK1 and ERK2 activation loop.
Sequence:
HTGFLpTEpYVAT

Run BLAST with Run BLAST with
Epitope

The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2).
Positive control
- Rat brain extract, Drosophila wing imaginal disc.
General notes

This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot is from ascites and is still in stock as of 25th October 2016- GR185138. Lot numbers higher than GR185138 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Application	Species
ICC/IF	Rat Drosophila melanogaster
WB	Rat

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS
Concentration information loading...
Purity

Proprietary Purification
Purification notes

Purified from tissue culture supernatant.
Clonality

Monoclonal
Clone number

MAPK-YT
Isotype

IgG1
Research areas

Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)

Lane 1 : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011) at 1/10000 dilution
Lane 2 : no primary antibody

All lanes : Extract of Rat1 cells activated with 100µM
Vanadate+200µM H₂O₂

Secondary
All lanes : Goat Anti-Mouse IgG, AlkPhos
Conjugate

Predicted band size: 44 kDa
Observed band size: 42,44 kDa
why is the actual band size different from the predicted?
Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)

In situ immunostaining of the activated form of MAP kinase with ab50011 (green) in Drosophila wing imaginal disc (500 micrometers long).
Immunocytochemistry/ Immunofluorescence - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody [MAPK-YT] (ab50011)

ICC/IF of NRK cells labeling the activated form of MAP kinase with ab50011 (1/1000 dilution). The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue).

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