Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Neuroscience Neurotransmission Intracellular Signaling Kinases

Anti-BRAF antibody (ab85972)

Price and availability

291 484 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-BRAF antibody (ab85972)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to BRAF
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Product image
Anti-CrkL antibody [Y244] - BSA and Azide free (ab247210)
Product image
Human PAK2 knockout HeLa cell pellet (ab278854)
Product image
Human MAP3K14 (NFkB Inducing Kinase NIK) knockout A549 cell line (ab267221)
Product image
Anti-TrkB antibody - BSA and Azide free (Detector) (ab242343)

Overview

  • Product name

    Anti-BRAF antibody
    See all BRAF primary antibodies
  • Description

    Rabbit polyclonal to BRAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human BRAF aa 25-75.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab85972 was affinity purified using an epitope specific to B Raf immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Kinases
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Signal transducers

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody (ab85972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRAF antibody (ab85972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling B Raf with ab85972 at 1/200 (1µg/ml). Detection: DAB.
  • Western blot - Anti-BRAF antibody (ab85972)
    Western blot - Anti-BRAF antibody (ab85972)
    All lanes : Anti-BRAF antibody (ab85972) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate

    at 50 µg
    Lane 2 : HeLa whole cell lysate

    at 15 µg
    Lane 3 : HeLa whole cell lysate

    at 5 µg
    Lane 4 : 293T whole cell lysate

    at 50 µg

    Predicted band size: 85 kDa
    Observed band size: 95 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 238 kDa, 60 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-BRAF antibody (ab85972)
    Immunoprecipitation - Anti-BRAF antibody (ab85972)
    Detection of B Raf by Western Blot of Immunprecipitate.
    ab85972 at 1µg/ml staining B Raf in HeLa whole cell lysate immunoprecipitated using ab85972 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-BRAF antibody (ab85972)

  •  
  • Product image

    Anti-BRAF (mutated V600E) antibody [RM8] (ab200535)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-BRAF antibody [EP152Y] (ab33899)

    Applications: IHC-P, IP, WB

  •  
  • Product image

    Anti-BRAF (phospho T401) antibody [EPR2208Y] (ab68215)

    Applications: WB

  •  
  • Product image

    Anti-BRAF (mutated V600E) antibody [VE1] (ab228461)

    Applications: IHC-P

  •  
  • Product image

    Anti-BRAF (phospho S729) antibody [EPR2207] (ab124794)

    Applications: WB

  •  
  • Product image

    Anti-BRAF antibody [pBR1] (ab183337)

    Applications: IHC-P

  •  
  • Product image

    Anti-BRAF antibody [EP152Y] - BSA and Azide free (ab189351)

    Applications: IHC-P, IP, WB

Clear all

Recently viewed products

  •  
  • Product image

    Anti-SNAP25 antibody [EP3274] (ab108990)

  •  
  • Product image

    Anti-SLP76 antibody [EPR2549(2)] (ab109254)

  •  
  • Product image

    Anti-FNDC3A antibody (ab254706)

  •  
  • Product image

    Anti-Tm9sf3 antibody (ab150587)

  •  
  • Product image

    Anti-FZD3 antibody (ab217032)

  •  
  • Product image

    Anti-CBP80 antibody (ab42389)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.