Anti-TRPM7 antibody (ab729)
Key features and details
- Goat polyclonal to TRPM7
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRPM7 antibody
See all TRPM7 primary antibodies -
Description
Goat polyclonal to TRPM7 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide: TKESESTNSVRLML, corresponding to C terminal amino acids 1852-1865 of Human TRPM7.
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General notes
In response to customer feedback and in-house testing the latest lot doesn't pass the WB quality testing so we have decided to remove the application for now. Please contact technical@abcam.com for more details.
Please see reference He et al for details of the size of the protein detected by SDS-PAGE. Principal Names: TRPM7; transient receptor potential cation channel, subfamily M, member 7; CHAK; CHAK1; LTRPC7; FLJ20117; TRP-PLIK; LTRPC ion channel family member 7; homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein. Official Gene Symbol - TRPM7 GenBank Accession Number – XP_030709 LocusLink ID - 54822 (human).
Can be blocked with Human TRPM7 peptide (ab22777).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-TRPM7 antibody (ab729)
Immunocytochemistry/ Immunofluorescence analysis of U2OS labeling TRPM7 with ab729 at 10 ug/mL. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 antibody was used as the secondary antibody (2ug/mL). Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
