Anti-HDAC7 antibody (ab23657)
Key features and details
- Rabbit polyclonal to HDAC7
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC7 antibody
See all HDAC7 primary antibodies -
Description
Rabbit polyclonal to HDAC7 -
Host species
Rabbit -
Specificity
ab23657 recognises a band of 105 kDa in NIH 3T3 cell lysates, and 103 kDa in human adult and fetal heart tissue lysates (see image). However, ab23657 does not appear to recognise HDAC7 in human thymus or CHO-K1 cell lysates, which may be due to low expression in these cells (data not shown). -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to Human HDAC7 aa 900 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab27456) -
Positive control
- This antibody gave a positive signal in the NIH3T3 whole cell lysate and in Human Heart - fetal and adult normal tissue lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23657 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseHumanApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsMay be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. -
Cellular localization
Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 51564 Human
- Entrez Gene: 56233 Mouse
- Omim: 606542 Human
- SwissProt: Q8WUI4 Human
- SwissProt: Q8C2B3 Mouse
- Unigene: 200063 Human
- Unigene: 384027 Mouse
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Alternative names
- DKFZP586J0917 antibody
- FLJ99588 antibody
- HD 7a antibody
see all
Images
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Western blot - Anti-HDAC7 antibody (ab23657)All lanes : Anti-HDAC7 antibody (ab23657) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Heart (Human) Tissue Lysate - fetal normal tissue
Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 103,105 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 70 kDa, 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab23657 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This antibody was raised against an immunogen that is predicted to recognize multiple isoforms of HDAC7.
Datasheets and documents
References (0)
ab23657 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-HDAC7 antibody (ab23657)All lanes : Anti-HDAC7 antibody (ab23657) at 1 µg/ml
Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 2 : Heart (Human) Tissue Lysate - fetal normal tissue
Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 103 kDa
Observed band size: 103,105 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa, 70 kDa, 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab23657 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
This antibody was raised against an immunogen that is predicted to recognize multiple isoforms of HDAC7.
