Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSN7b antibody [EPR6465] (ab124718)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6465] to CSN7b
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CSN7b antibody [EPR6465]
    See all CSN7b primary antibodies

  • Description

    Rabbit monoclonal [EPR6465] to CSN7b

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CSN7b aa 200-300. The exact sequence is proprietary.

  • Positive control

    • WB: HEK293T, HAP1, Jurkat, HeLa, HL-60, and HT-29 cell lysates. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)
    Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)
    All lanes : Anti-CSN7b antibody [EPR6465] (ab124718) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : COPS7B knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HAP1 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 30 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab124718 observed at 32 kDa. Red - loading control ab8245 observed at 36 kDa. 

     ab124718 Anti-CSN7b antibody [EPR6465]  was shown to specifically react with CSN7b in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266646 (knockout cell lysate ab257895) was used.  Wild-type and CSN7b knockout samples were subjected to SDS-PAGE.  ab124718 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)
    Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CSN7b knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab124718 observed at 32 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab124718 was shown to specifically react with CSN7b when CSN7b knockout samples were used. Wild-type and CSN7b knockout samples were subjected to SDS-PAGE. ab124718 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-CSN7b antibody [EPR6465] (ab124718)
    Flow Cytometry - Anti-CSN7b antibody [EPR6465] (ab124718)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling CSN7b with unpurified ab124718 at 1/800 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)
    Western blot - Anti-CSN7b antibody [EPR6465] (ab124718)
    All lanes : Anti-CSN7b antibody [EPR6465] (ab124718) at 1/10000 dilution

    Lane 1 : Jurkat cell lysates
    Lane 2 : HeLa cell lysates
    Lane 3 : HL60 cell lysates
    Lane 4 : HT-29 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CSN7b antibody [EPR6465] (ab124718)
    Immunocytochemistry/ Immunofluorescence - Anti-CSN7b antibody [EPR6465] (ab124718)
    ab124718, at 1/50, staining CSN7b in HeLa cells by Immunofluorescence.
  • Anti-CSN7b antibody [EPR6465] (ab124718)
    Anti-CSN7b antibody [EPR6465] (ab124718)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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