Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CrkLY207-G4] to CrkL (phospho Y207)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CrkL (phospho Y207) antibody [CrkLY207-G4]
    See all CrkL primary antibodies

  • Description

    Rabbit monoclonal [CrkLY207-G4] to CrkL (phospho Y207)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CrkL (phospho Y207). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P46109

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: K562 and NIH/3T3 cell extracts; Mouse E10.5 whole embryo extract. Flow cyt: K562 cells (treated with pervanadate).

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Flow Cytometry - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Flow Cytometry - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using 0.05 µg/mL of ab278698 at 0.1ug/mL.
  • Western blot - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Western blot - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    All lanes : Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698) at 1/500 dilution (Green)

    Lane 1 : E10.5 mouse Crkl knock out (-/-) whole embryos.
    Lane 2 : E10.5 mouse wild-type (+/+) whole embryos.

    Predicted band size: 34 kDa



    The red channel was stained using a B-actin loading control.

  • Western blot - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Western blot - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698) at 1 µg/ml + NIH3T3 cell extract

    Predicted band size: 34 kDa

  • Flow Cytometry - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Flow Cytometry - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Peptide blocking flow cytometric analysis of K562 cells secondary antibody only negative control (light blue) or treated with imatinib (red) or treated with pervanadate (green) or imatinib and blocked with phospho-peptide (black) or pervanadate and blocked with phospho peptide (gold) or imatinib and blocked with non-phospho peptide (dark blue) or pervanadate and blocked with non-phospho peptide (purple) using ab278698 at 0.01µg/mL.
  • Western blot - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Western blot - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    All lanes : Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698) at 0.001 µg/ml

    Lane 1 : K562 cell extract untreated
    Lane 2 : K562 cell extract treated with imatinib

    Predicted band size: 34 kDa

  • Flow Cytometry - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Flow Cytometry - Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Flow cytometric analysis of K562 cells secondary antibody only negative control (blue) or imatinib (red) or treated with pervanadate (green) using ab278698 at 0.05 µg/mL. .
  • Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)
    Anti-CrkL (phospho Y207) antibody [CrkLY207-G4] (ab278698)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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