Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)
![Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)](https://www.abcam.com/ps/products/264/ab264052/Images/ab264052-448452-anti-idh2-mutated-r172-k-antibody-r511kmab1-western-blot-histagged-human-idh1.jpg "Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [R511-KMab-1] to IDH2 (mutated R172K)
- Suitable for: ELISA, WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-IDH2 (mutated R172K) antibody [R511-KMab-1]
See all IDH2 primary antibodies -
Description
Rat monoclonal [R511-KMab-1] to IDH2 (mutated R172K) -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
R511-KMab-1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)
ab264052 used at 1/1000 dilution (0.805 μg/ml).
Secondary used: Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10,000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
The loading samples are E.coil extracts containing recombinant protein respectively.
Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng
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![ELISA - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)](https://www.abcam.com/ps/products/264/ab264052/Images/ab264052-448453-AntiIDH2mutatedR172KantibodyR511KMab1ELISAhuman.jpg)
ELISA - Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)ab264052 used at 0-5000 ng/ml.
Antigen (IDH2, IDH2(R172G), IDH2(R172M), IDH2(R172S), IDH2(R172W), IDH2(R172K), IDH2(R140Q)) used at 100 ng/ml.
Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.
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![Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)](https://www.abcam.com/ps/products/264/ab264052/Images/ab264052-456264-benefitsofrecombinantantibodies.jpg)
Anti-IDH2 (mutated R172K) antibody [R511-KMab-1] (ab264052)