Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)
Key features and details
- Rabbit polyclonal to STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody -
Description
Rabbit polyclonal to STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) -
Host species
Rabbit -
Specificity
ab79199 detects endogenous levels of STK3/MST1 only when phosphorylated at threonine 183 (MST1) and/or threonine T180 (STK3). -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide (phospho T183). Synthetic phosphopeptide derived from human STK3/MST1 around the phosphorylation site of threonine 183 (R-N-TP-V-I).
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Positive control
- WB: Extracts from 293 cells treated with H2O2 (100uM, 15mins). IHC-P: Human liver carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab79199 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)All lanes : Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)
Lane 1 : Extracts from 293 cells, treated
with H2O2 (100uM, 15mins)
Lane 2 : Extracts from 293 cells, treated
with H2O2 (100uM, 15mins) with immunising phosphopeptide
Predicted band size: 56 kDa
Observed band size: 56 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK3/MST-2 (phospho T180) + MSP/MST1 (phospho T183) antibody (ab79199)ab79199, at 1/50 dilution, staining STK3 (phospho T180) + MSP/MST1 (phospho T183) in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.