Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9432] to PRDM4/PFM1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PRDM4/PFM1 antibody [EPR9432]
    See all PRDM4/PFM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR9432] to PRDM4/PFM1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human PRDM4/PFM1 (Q9UKN5).

  • Positive control

    • HeLa, 293T, PC3 and SH SY5Y cell lysates; Human kidney tissue; HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : PC-3 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa



    Blocking buffer: 5% NFDM/TBST.

  • Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution

    Lane 1 : Mouse heart tissue lysate
    Lane 2 : Rat heart tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H + L) at 1/1000 dilution

    Predicted band size: 88 kDa
    Observed band size: 88 kDa



    Blocking buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)

    Immunocytochemistry/Immunofluorescence analysis of 293 cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.

  • Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
    Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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