Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
![Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)](https://www.abcam.com/ps/products/156/ab156867/Images/ab156867-220966-anti-prdm4-pfm1-antibody-epr9432-immunohistochemistry.jpg "Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9432] to PRDM4/PFM1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-PRDM4/PFM1 antibody [EPR9432]
See all PRDM4/PFM1 primary antibodies -
Description
Rabbit monoclonal [EPR9432] to PRDM4/PFM1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB MouseHuman
-
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues in Human PRDM4/PFM1 (Q9UKN5).
-
Positive control
- HeLa, 293T, PC3 and SH SY5Y cell lysates; Human kidney tissue; HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9432 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)](https://www.abcam.com/ps/products/156/ab156867/Images/ab156867-220968-anti-prdm4-pfm1-antibody-epr9432-western-blot.jpg)
Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : PC-3 cell lysate
Lane 4 : SH-SY5Y cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDaBlocking buffer: 5% NFDM/TBST.
-
![Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)](https://www.abcam.com/ps/products/156/ab156867/Images/ab156867-220969-anti-prdm4-pfm1-antibody-epr9432-western-blot.jpg)
Western blot - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)All lanes : Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H + L) at 1/1000 dilution
Predicted band size: 88 kDa
Observed band size: 88 kDaBlocking buffer: 5% NFDM/TBST.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)](https://www.abcam.com/ps/products/156/ab156867/Images/ab156867-220967-anti-prdm4-pfm1-antibody-epr9432-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue labelling PRDM1/PFM1 with ab156867 at 1/500. An ImmunoHistoprobe (Ready to use) HRP polymer for Rabbit IgG was used as the secondary antibody. Tissue was counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
-
![Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)](https://www.abcam.com/ps/products/156/ab156867/Images/ab156867-220964-anti-prdm4-pfm1-antibody-epr9432-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.
-
![Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)](https://www.abcam.com/ps/products/156/ab156867/Images/ab156867-220965-anti-prdm4-pfm1-antibody-epr9432-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)Immunocytochemistry/Immunofluorescence analysis of 293 cells labelling PRDM4/PFM1 with ab156867 at 1/250. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugtaed goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI.
-
![Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)](https://www.abcam.com/ps/products/156/ab156867/Images/ab156867-10-benefits-of-recombinant-antibodies.png)
Anti-PRDM4/PFM1 antibody [EPR9432] (ab156867)
