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Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet (ab278843)

Overview

  • Product name

    Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet
    See all KMT3A / HYPB / HIF-1 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 3 and 4 bp deletion in exon 3.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Belongs to the histone-lysine methyltransferase family. SET2 subfamily.
    Contains 1 AWS domain.
    Contains 1 post-SET domain.
    Contains 1 SET domain.
    Contains 1 WW domain.

  • Domain

    The low charge region mediates the transcriptional activation activity.

  • Post-translational
    modifications

    May be automethylated.

  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt

  • Alternative names

    • EC 2.1.1.43
    • FLJ16420
    • FLJ22472
    • FLJ23184
    • FLJ45883
    • HBP231
    • HIF 1
    • HIF-1
    • HIF1
    • HIP-1
    • Histone lysine N methyltransferase SETD2
    • Histone-lysine N-methyltransferase SETD2
    • hSET2
    • HSPC069
    • Huntingtin interacting protein
    • Huntingtin interacting protein 1
    • Huntingtin interacting protein B
    • Huntingtin interacting protein HYPB
    • Huntingtin yeast partner B
    • Huntingtin-binding protein, 231-KD
    • Huntingtin-interacting protein 1
    • Huntingtin-interacting protein B
    • HYPB
    • KIAA1732
    • KMT3A
    • Lysine N methyltransferase 3A
    • Lysine N-methyltransferase 3A
    • p231HBP
    • SET domain containing 2
    • SET domain-containing protein 2
    • SET2
    • SETD2
    • SETD2_HUMAN
    see all

Properties

Images

  • Western blot - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet (ab278843)
    Western blot - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet (ab278843)

    Lane 1: Wild-type HEK293T cell lysate (20 µg)

    Lane 2: SETD2 knockout HEK293T cell lysate (20 µg)

    Lane 3: HepG2 cell lysate (20 µg)

    Lanes 1-3: Merged signal (red and green). Green - ab31358 observed at 288 kDa. Red - loading control, ab7291 observed at 50 kDa.

    ab31358 Anti-KMT3A / HYPB / HIF-1 antibody was shown to specifically react with KMT3A / HYPB / HIF-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266690 (knockout cell lysate ab257274) was used. Wild-type and KMT3A / HYPB / HIF-1 knockout samples were subjected to SDS-PAGE. ab31358 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti- Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti- Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet (ab278843)
    Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet (ab278843)

    Allele-1: 11 bp deletion in exon 3

     

  • Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet (ab278843)
    Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HEK293T cell pellet (ab278843)

    Allele-2: 4 bp deletion in exon 3

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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