Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18875] to KDM3A / JHDM2A - BSA and Azide free
  • Suitable for: IP, WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free
    See all KDM3A / JHDM2A primary antibodies

  • Description

    Rabbit monoclonal [EPR18875] to KDM3A / JHDM2A - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human KDM3A/ JHDM2A aa 750-850. The exact sequence is proprietary.
    Database link: Q9Y4C1

  • General notes

    Ab251059 is the carrier-free version of ab191389. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251059 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)

    This data was developed using ab191389, the same antibody clone in a different buffer formulation.

    Lane 1: Wild type HAP1 whole cell lysate (40 µg)

    Lane 2: KDM3A knockout HAP1 whole cell lysate (40 µg)

    Lane 3: HeLa whole cell lysate (40 µg)

    Lane 4: Hek293 whole cell lysate (40 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab191389 observed at 150 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab191389 was shown to specifically react with KDM3A when KDM3A knockout samples were used. Wild-type and KDM3A knockout samples were subjected to SDS-PAGE. ab191389 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 500 dilution and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    All lanes : Anti-KDM3A / JHDM2A antibody [EPR18875] (ab191389) at 1/1000 dilution

    Lane 1 : Untreated HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) treated with 100 μM CoCl2 for 24 hours whole cell lysate
    Lane 3 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with 100 μM CoCl2 for 24 hours whole cell lysate
    Lane 5 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) treated with 100 μM CoCl2 for 24 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000 mg/ml

    Predicted band size: 147 kDa
    Observed band size: 100,147,75 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab191389, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1 and 2: 15 seconds; Lanes 3 and 4: 30 seconds; Lanes 5 and 6: 5 seconds.

  • Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Western blot - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Anti-KDM3A / JHDM2A antibody [EPR18875] (ab191389) at 1/1000 dilution + Ramos (Human Burkitt's lymphoma cell line) whole cell lysate

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 147 kDa
    Observed band size: 147 kDa


    Exposure time: 3 minutes


    This data was developed using ab191389, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    This data was developed using ab191389, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KDM3A / JHDM2A with ab191389 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on spermatogenic cells of human testis is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    This data was developed using ab191389, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling KDM3A / JHDM2A with ab191389 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Weak nuclear staining on cancer cells of human bladder cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    This data was developed using ab191389, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling KDM3A / JHDM2A with ab191389 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Weak nuclear staining on cancer cells of human lung cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Immunoprecipitation - Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    This data was developed using ab191389, the same antibody clone in a different buffer formulation.KDM3A / JHDM2A was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell lysate with ab191389 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab191389 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: Ramos whole cell lysate 10µg (Input). Lane 2: ab191389 IP in Ramos whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191389 in Ramos whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)
    Anti-KDM3A / JHDM2A antibody [EPR18875] - BSA and Azide free (ab251059)

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