Human SOX2 ELISA Kit (ab245707)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 31 pg/ml
- Range: 0.46875 ng/ml - 30 ng/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SOX2 ELISA Kit
See all SOX2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% cell extract 24 2.7% Inter-assay Sample n Mean SD CV% cell extract 3 4.1% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
31 pg/ml -
Range
0.469 ng/ml - 30 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 88 79% - 98% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human SOX2 ELISA Kit (ab245707) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SOX2 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SOX2 with 31 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human SOX2 Capture Antibody 1 x 600µl 10X Human SOX2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human SOX2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. -
Involvement in disease
Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Post-translational
modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- ANOP3
- cb236
- Delta EF2a
see all -
Database links
- Entrez Gene: 6657 Human
- Omim: 184429 Human
- SwissProt: P48431 Human
- Unigene: 518438 Human
Images
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Other - Human SOX2 ELISA Kit (ab245707)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human SOX2 standard curve in 1X Cell Extraction Buffer PTR.The SOX2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native SOX2 in human NCCIT cell extracts based on a 50 µg/mL extract load.The concentrations of SOX2 were measured in duplicate and interpolated from the SOX2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SOX2 concentration was determined to be 36.48 ng/mL. -
Sandwich ELISA - Human SOX2 ELISA Kit (ab245707)To learn more about the advantages of recombinant antibodies see here.
