Anti-CD133 antibody [BLR093G] (ab276130)
![Anti-CD133 antibody [BLR093G] (ab276130)](https://www.abcam.com/ps/products/276/ab276130/Images/ab276130-412253-AntiCD133-antibody-BLR093G-westernblot-HEK293T-SW620-GaMG-OVCAR4-U2OS-HepG2-A549-and-MCF7-cells.JPG "Anti-CD133 antibody [BLR093G] (ab276130)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR093G] to CD133
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
Overview
-
Product name
Anti-CD133 antibody [BLR093G]
See all CD133 primary antibodies -
Description
Rabbit monoclonal [BLR093G] to CD133 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human
-
Immunogen
Synthetic peptide within Human CD133 aa 350-400. The exact sequence is proprietary. NP_006008.1
Database link: O43490 -
Positive control
- WB: SW620, OVCAR-4, and HepG2 cells. IHC-P: Human colon carcinoma, ovarian carcinoma. ICC: OVCAR-4 cells, SW620 cells
-
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 98% Borate buffered saline -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
BLR093G -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-CD133 antibody [BLR093G] (ab276130)All lanes : Anti-CD133 antibody [BLR093G] (ab276130) at 1/1000 dilution
Lane 1 : HEK-293T whole cell lysate
Lane 2 : SW620 whole cell lysate
Lane 3 : GaMG whole cell lysate
Lane 4 : OVCAR-4 whole cell lysate
Lane 5 : U-2 OS whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : A549 whole cell lysate
Lane 8 : MCF7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Predicted band size: 97 kDaChemiluminescence with an exposure time of 3 minutes.
Lower Panel: Rabbit anti-COPB2 antibody.
-
![Immunocytochemistry - Anti-CD133 antibody [BLR093G] (ab276130)](https://www.abcam.com/ps/products/276/ab276130/Images/ab276130-412252-AntiCD133-antibody-BLR093G-immunocytochemistry-sw620-cells-human.JPG)
Immunocytochemistry - Anti-CD133 antibody [BLR093G] (ab276130)SW620 cells stained for CD133 using ab276130 at 1/500 dilution in immunocytochemical analysis
-
![Immunocytochemistry - Anti-CD133 antibody [BLR093G] (ab276130)](https://www.abcam.com/ps/products/276/ab276130/Images/ab276130-412251-AntiCD133-antibody-BLR093G-immunocytochemistry-ovar4-cells-human.JPG)
Immunocytochemistry - Anti-CD133 antibody [BLR093G] (ab276130)OVCAR-4 cells stained for CD133 using ab276130 at 1/500 dilution in immunocytochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (ab276130)](https://www.abcam.com/ps/products/276/ab276130/Images/ab276130-412250-AntiCD133-antibody-BLR093G-immunohistochemistry-ovarian-carcinoma-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (ab276130)Paraffin embedded human colon carcinoma tissue stained for CD133 using ab276130 at 1/500 dilution in immunohistochemical analysis.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (ab276130)](https://www.abcam.com/ps/products/276/ab276130/Images/ab276130-412249-AntiCD133-antibody-BLR093G-immunohistochemistry-coloncarcinoma-human.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (ab276130)Paraffin embedded human colon carcinoma tissue stained for CD133 using ab276130 at 1/500 dilution in immunohistochemical analysis.
-
![Anti-CD133 antibody [BLR093G] (ab276130)](https://www.abcam.com/ps/products/276/ab276130/Images/ab276130-6-benefits-of-recombinant-antibodies.png)
Anti-CD133 antibody [BLR093G] (ab276130)