Antibodies, Proteins, Kits and Reagents for Life Science

Anti-CD133 antibody [BLR093G] (ab276130)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR093G] to CD133
Suitable for: WB, IHC-P, ICC
Reacts with: Human

Product name

Anti-CD133 antibody [BLR093G]
See all CD133 primary antibodies
Description

Rabbit monoclonal [BLR093G] to CD133
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD133 aa 350-400. The exact sequence is proprietary. NP_006008.1
Database link: O43490
Positive control
- WB: SW620, OVCAR-4, and HepG2 cells. IHC-P: Human colon carcinoma, ovarian carcinoma. ICC: OVCAR-4 cells, SW620 cells
General notes

This product is sold under License from Bethyl Laboratories, Inc.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 8.20
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, 98% Borate buffered saline
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

BLR093G
Isotype

IgG
Research areas

Western blot - Anti-CD133 antibody [BLR093G] (ab276130)

All lanes : Anti-CD133 antibody [BLR093G] (ab276130) at 1/1000 dilution

Lane 1 : HEK-293T whole cell lysate
Lane 2 : SW620 whole cell lysate
Lane 3 : GaMG whole cell lysate
Lane 4 : OVCAR-4 whole cell lysate
Lane 5 : U-2 OS whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : A549 whole cell lysate
Lane 8 : MCF7 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 97 kDa

Chemiluminescence with an exposure time of 3 minutes.

Lower Panel: Rabbit anti-COPB2 antibody.
Immunocytochemistry - Anti-CD133 antibody [BLR093G] (ab276130)

SW620 cells stained for CD133 using ab276130 at 1/500 dilution in immunocytochemical analysis
Immunocytochemistry - Anti-CD133 antibody [BLR093G] (ab276130)

OVCAR-4 cells stained for CD133 using ab276130 at 1/500 dilution in immunocytochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (ab276130)

Paraffin embedded human colon carcinoma tissue stained for CD133 using ab276130 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD133 antibody [BLR093G] (ab276130)

Paraffin embedded human colon carcinoma tissue stained for CD133 using ab276130 at 1/500 dilution in immunohistochemical analysis.
Anti-CD133 antibody [BLR093G] (ab276130)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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