Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Hamartin antibody [OTI3A2] (ab170164)

Key features and details

Mouse monoclonal [OTI3A2] to Hamartin
Suitable for: WB, IHC-P
Reacts with: Human
Isotype: IgG2a

Product name

Anti-Hamartin antibody [OTI3A2]
See all Hamartin primary antibodies
Description

Mouse monoclonal [OTI3A2] to Hamartin
Host species

Mouse
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human Hamartin aa 800 to the C-terminus. Produced in E.coli. NP_000359
Database link: Q92574

Run BLAST with Run BLAST with
Positive control
- HEK293T cell lysate transfected with pCMV6-ENTRY Hamartin cDNA; Human breast, colon, colon adenocarcinoma, pancreas, endometrium adenocarcinoma, prostate and prostate carcinoma tissues.
General notes

The clone number has been updated from 3A2 to OTI3A2, both clone numbers name the same clone.

This product was changed from ascites to tissue culture supernatant on 10^th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Purity

Protein A purified
Purification notes

ab170164 is purified from TCS by affinity chromatography.
Clonality

Monoclonal
Clone number

OTI3A2
Isotype

IgG2a
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human prostate carcinoma tissue labeling Hamartin with ab170164 at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hamartin antibody [OTI3A2] (ab170164)

Immunohistochemical analysis of paraffin-embedded Human adult heart tissue tissue labeling Hamartin with ab170164 at 1/150 dilution.
Western blot - Anti-Hamartin antibody [OTI3A2] (ab170164)

All lanes : Anti-Hamartin antibody [OTI3A2] (ab170164) at 1/2000 dilution

Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY Hamartin cDNA

Lysates/proteins at 5 µg per lane.

Predicted band size: 130 kDa

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC218987)

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