Goat Anti-Human IgG H&L (HRP) (ab81202)
Key features and details
- Goat Anti-Human IgG H&L (HRP)
- Conjugation: HRP
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ELISA
Overview
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Product name
Goat Anti-Human IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Human -
Specificity
No crossreaction with human IgM and IgA in ELISA No crossreaction with albumin fraction of human serum. No crossreaction with albumin fraction of other animal species. -
Tested applications
Suitable for: WB, ELISAmore details -
Immunogen
Human IgG.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.50
Constituents: 50% Glycerol, 0.58% Sodium chloride, 0.164% Sodium phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
From the crude polyclonal the crossreactive antibodies were extracted by incubation with Sepharose bound human IgA and IgM. Specific antibodies were absorpted by incubation with Sepharose bound human IgG. Specific antibodies were eluted by acidic buffer at pH 2.5 followed by neutralization and dialysis. After repeated binding with immobilized human IgG a minimum of 65% protein bound. -
Conjugation notes
The purified polyclonal was conjugated to horseradish peroxidase according to the periodate method followed by gel filtation and ion exchange chromatography to clear unbound conjugate. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas