Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Human IgG3 antibody [RM119] (ab193172)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM119] to Human IgG3
  • Suitable for: Sandwich ELISA, IHC-P, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Human IgG3 antibody [RM119]
    See all Human IgG3 primary antibodies

  • Description

    Rabbit monoclonal [RM119] to Human IgG3

  • Host species

    Rabbit

  • Specificity

    Reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    ICC
    Human
    See all applications and species data

  • Immunogen

    Full length protein corresponding to Human Human IgG3. Purified Native Human IgG3 from Human Plasma
    Database link: P01860

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Protein A affinity purified from an animal origin–free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM119

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG3 antibody [RM119] (ab193172)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG3 antibody [RM119] (ab193172)

    Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section labeling IgG3 with ab193172.

  • ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)
    ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)

    A titer ELISA using ab193172. The plate was coated with different amounts of human IgG3. A serial dilution of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)
    ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)

    ELISA of human immunoglobulins shows ab193172 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)
    Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)

    Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

  • Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)
    Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)

    Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

  • Anti-Human IgG3 antibody [RM119] (ab193172)
    Anti-Human IgG3 antibody [RM119] (ab193172)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Human IgG3 antibody [RM119] (ab193172)

© 2021 Astana Biomed Group LLP. All rights reserved.