Anti-Human IgG3 antibody [RM119] (ab193172)
![Anti-Human IgG3 antibody [RM119] (ab193172)](https://www.abcam.com/ps/products/193/ab193172/Images/ab193172-456631-anti-human-Igg3-antibody-immunohistochemistry-lymphoid-human.jpg "Anti-Human IgG3 antibody [RM119] (ab193172)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM119] to Human IgG3
- Suitable for: Sandwich ELISA, IHC-P, ELISA
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Human IgG3 antibody [RM119]
See all Human IgG3 primary antibodies -
Description
Rabbit monoclonal [RM119] to Human IgG3 -
Host species
Rabbit -
Specificity
Reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. -
Tested Applications & Species
See all applications and species dataApplication Species ELISA Recombinant fragmentICC Human
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Immunogen
Full length protein corresponding to Human Human IgG3. Purified Native Human IgG3 from Human Plasma
Database link: P01860
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 48% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Protein A affinity purified from an animal origin–free culture supernatant. -
Clonality
Monoclonal -
Clone number
RM119 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG3 antibody [RM119] (ab193172)
Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section labeling IgG3 with ab193172.
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![ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)](https://www.abcam.com/ps/products/193/ab193172/Images/ab193172-230564-anti-human-igg3-antibody-rm119-elisa.jpg)
ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)A titer ELISA using ab193172. The plate was coated with different amounts of human IgG3. A serial dilution of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)](https://www.abcam.com/ps/products/193/ab193172/Images/ab193172-230563-anti-human-igg3-antibody-rm119-elisa.jpg)
ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)ELISA of human immunoglobulins shows ab193172 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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![Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)](https://www.abcam.com/ps/products/193/ab193172/Images/ab193172-230561-anti-human-igg3-antibody-rm119-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.
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![Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)](https://www.abcam.com/ps/products/193/ab193172/Images/ab193172-230560-anti-human-igg3-antibody-rm119-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (ab193172)Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.
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![Anti-Human IgG3 antibody [RM119] (ab193172)](https://www.abcam.com/ps/products/193/ab193172/Images/ab193172-5-benefits-of-recombinant-antibodies.png)
Anti-Human IgG3 antibody [RM119] (ab193172)
