Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Histone H3 (di-methyl K9) Quantification Kit (Colorimetric)
See all Histone H3 kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Assay time
2h 30m -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Mammals
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Product overview
Methylation of histone H3 at lysine 9 by ESET, G9a among other methyl transferases is involved in euchromatin gene silencing as well as in heterochromatin formation. Specifically, H3 (di-methyl K9) mediates heterochromatin formation by forming a binding site for HP1.
Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062) allows the user to specifically measure global di-methylation of histone H3K9 colorimetrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
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Platform
Microplate reader
Properties
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Storage instructions
Please refer to protocols. -
Components Identifier 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with frame) 4 units 9 units 8-Well Standard Control Strips Green Ringed 2 units 3 units Antibody Buffer 1 x 6ml 1 x 2ml Color Developer 1 x 5ml 1 x 10ml Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Standard Control (100 µg/mL) 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml -
Research areas
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Relevance
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Cellular localization
Nucleus. Chromosome. -
Alternative names
- H3f3a
- H3FA
- H3FB
see all -
Database links
- Entrez Gene: 8350 Human
- Entrez Gene: 8351 Human
- Entrez Gene: 8352 Human
- Entrez Gene: 8353 Human
- Entrez Gene: 8354 Human
- Entrez Gene: 8355 Human
- Entrez Gene: 8356 Human
- Entrez Gene: 8357 Human
see all
Images
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Functional Studies - Histone H3 (di-methyl K9) Quantification Kit (Colorimetric) (ab115062)
Histone extracts were prepared from MDA-MB-231 cells using the Histone Extraction Kit (ab113476) and the amount of dimethyl-H3-K9 was measured using ab115062.
